Study On The Inhibitory Action Of HDACI On Choriocarcinoma Cell JAR Lines In Vitro

Aims:To measure the survival ratio,inhibition ratio and apoptisis ratio in choriocarcinoma cell lines JAR cultured in vitro after using different concentrations of VPA and VPA+5-FU,try to prove VPA can inhibit cell's growth,induce apoptosis and enhance anticancer response of 5-FU.Methods:Choriocarcinoma cell lines JAR cultured in vitro were used in study.JAR was treated respectively in seven different concentrations of VPA,5-FU,VPA+5-FU for 48 hours later.Then MTT method was used to measured OD,which was an indirect response to cell survival and inhibition ratio,by the micro plate reader.Calculated the IC50 via the method of SPSS.Then cultured the cells in the different drugs that concentration was IC50 for 48 hours later,measured apoptosis ratio of different cells by flow cytometry.Results:(1) The inhibition actions of JAR cell lines was significant correlation with concentration both in VPA group and VPA+5-FU group. (r=0.898,0.718,p＜0.05).And inhibitory effect of VPA+5-FU group is stronger than that of VPA.IC50(VPA)=2.695mmol/L,IC50(VPA+5-FU) =0.811mmol/L.(2) JAR cell lines could be induced to apoptosis or necrosis by VPA,The total apoptosis ratio induced by VPA or VPA+5-FU in JAR cell lines was significant different(p＞0.05),and the necrosis caused by VPA+5-FU in JAR cell lines was more than VPA.Conclusions:The results suggested that the role of VPA could inhibit the growth of JAR cell lines,and showed the dose-dependent manner.Addition,VPA could cause cell necrosis and apoptosis in JAR cell lines,terminal apoptosis significance.The third,VPA can Strengthen Antineoplastic 5-FU Function.