Effects Of NB-UVB Combined With Tacrolimus On Percutaneous Penetration Of Tacrolimus And Expression Of IL-10 In Mice

ObjectiveTo detect effects of NB-UVB associated with tacrolimus on the concentration of tacrolimus and the expression of IL-10 in mice, investigate effects of NB-UVB on the percutaneous penetration of tacrolimus and the combination of NB-UVB and tacrolimus to the immune function.Methods1. 24 Kunming mice were randomly divided into normal control group, tacrolimus group,tacrolimus plus NB-UVB group and NB-UVB plus tacrolimus group. Tacrolimus was applied twice daily,NB-UVB was applied once daily for 4 days,on the fifth day,mice were sacrificed 6 hours after single application of tacrolimus, HPLC -MS/MS was used to detect the concentration of tacrolimus in skin and blood.2. 30 BALB/c mice were randomly divided into normal control group,tacrolimus group,tacrolimus plus NB-UVB group,NB-UVB plus tacrolimus group and NB-UVB group, tacrolimus was applied twice daily,NB-UVB was applied once daily for 4 days,on the fifth day,mice were sacrificed 6 hours after single application of tacrolimus or one hour after NB-UVB radiation, ELISA was used to determine the IL-10 level in skin and blood.3. The data was expressed as mean±stand error. Data analysis was performed using SPSS 13.0 statistical package programme, a 0.05 level of probability was taken as the level of significance.Results1. There were significant difference of tacrolimus concentration in skin between NB-UVB associated with tacrolimus groups and tacrolimus group(P<0.05), there were no difference of tacrolimus concentration in skin between tacrolimus plus NB- UVB group and NB-UVB plus tacrolimus group(P>0.05), there were no difference of tacrolimus concentration in blood among the three groups(P>0.05), there were significant difference of tacrolimus concentration between skin and blood in the same group(P< 0.01).2. The IL-10 level of tacrolimus group,tacrolimus associatied with NB-UVB group,NB-UVB group all higher than normal control group both in skin and blood(P<0.05),there were no difference among other groups(P > 0.05),there were significant difference of IL-10 level between skin and blood in the same group(P<0.01).Conclusion1. NB-UVB can promote percutaneous penetration of tacrolimus and can elevate the concentration in skin,but has no effect on concentration in blood.2. The application of NB-UVB or tacrolimus and the combination of the two all can elevate IL-10 level in blood and skin, but combined application of tacrolimus and NB-UVB don't aggravate immunosuppression.3. The combination of NB-UVB and tacrolimus can elevate the therapeutical effect of tacrolimus,but don't aggravate immunosuppression of each other,providing evidence for combination of NB-UVB and tacrolimus.