Association Between Polymorphism Of Endothelial Nitric Oxide Synthase Gene And Type 2 Diabetic Patients With Erectile Dysfunction

Objective To investigate the relationship between the insert/deletion polymorphism of variable number of tandem repeat in intron 4(4b/a) or the single nucleotide polymorphism in exon 7 (G894T) of endothelial nitric oxide synthase(ecNOS) gene and type 2 diabetic patients with erectile dysfunction.Methods In 108 patients with type 2 diabetes mellitus(T2DM),94 type 2 diabetic patients with erectile dysfunction (DED) and 100 healthy controls(NC),The polymorphism of 4VNTR was determined by polymerase chain reaction-variable number of tandem repeat analysis(PCR),and the polymorphism of G894T was determined by polymerase chain reaction-restriction fragment length polymorphism analysis(PCR-RFLP).Results Smoking history and diabetic peripheral arterial disease(DPA) in DED group were higher than that of T2DM group(P﹤0.01).The concentration of LDL-C gradually increased from NC group,T2DM group to DED group ,repectively (2.37±0.86) mmol/L vs (2.85±0.74) mmol/L vs (3.12±0.92) mmol/L (P﹤0.05 or P﹤0.01).And the concentration of HbA1c gradually increased from NC group,T2DM group to DED group (6.40±0.64)% vs (5.06±1.09)% vs (7.32±0.66)% (P﹤0.05 or P﹤0.01) ).The concentration of TG in T2DM and DED group was higher than that of NC group, repectively (1.76±0.66) mmol/L, (1.89±0.52) mmol/L vs (1.35±0.54) mmol/L (P﹤0.01),and the concentration of TC in T2DM and DED group was higher than that of NC group,repectively (4.95±0.77) mmol/L,(5.06±1.09) mmol/L vs (4.31±0.86) mmol/L (P﹤0.01). The frequencies of allele 4a ,genotype containing with allele 4a (4b/a or 4a/a) and the frequencies of allele T ,genotype containing with allele T(GT or TT) in DED group were significantly higher than those in DM group or NC group (all P﹤0.01) . Logistic regression analysis showed that carriers of allele 4a ,allele T of ecNOS gene and diabetic peripheral arterial disease had a greater risk of diabetic erectile dysfunction. Conclusion The allele 4a ,allele T of ecNOS gene and diabetic peripheral arterial disease are the independent risk factors of type 2 diabetic patients with erectile dysfunction.