Primary Study In Castrated Rats Penile Corpus Cavernosum Smooth Muscle RyR1 And CaV1.3 Expression

To study the expression of ryanodine receptor1 and CaV1.3 in corpus cavernosum of castrated rats and investigate the mechanism of erectile dysfunction in relation to androgen deficiency. Methods : 40 SD rats of 8 weeks were randomly devided into:sham-operated 2 weeks group(A group), sham-operated 4 weeks group(B group),2 weeks castrated group(Cgroup),4 weesk castrated group(D group).To determine the serum testosterone level and using immunohistochemistry staining,RT-PCR to investigate the expression of ryanodine receptor1 and CaV1.3 in corpus cavernosum of castrated rats after 2 and 4 weeks operation respectively. Results: The serum testosterone in C,D group was significantly decreased as compared with A and B group(P<0.05). RyR1 and CaV1.3 express in all groups。RyR1mRNA,CaV1.3 mRNA and their proteins in C,D group was significantly decreased as compared with A and B group(P<0.05)。Androgen and RyR1,CaV1.3 related to each other. Conclusion: Androgen can modulate erectile function via RyR1 and CaV1.3.