Response Of Fatty Acids To Adiponectin Expression In Matured Adipocytes

Adipose tissue( AT) is a major metabolic organ. It can synthesizeand store triglyceride and release it when organ needs to maintain energy balance. Adipose tissue is now regarded as an important endocrine organ, secreting kinds of adipocytokines, such as leptin, TNF-a, IL-6, resistin and adiponectin. Adiponectin has been determined to have important role in insulin sensitizing, antiatherogenesis and antiinflammation. The expression of adiponectin decreases in obesity and insulin resistance. The increased level of Adiponectin is of great significance to improve the correlative disease state. 3T3-L1 preadipocytes were cultured and differentiated into the matured adipocytes in vitro. The differentiated adipocytes were incubated with different types of free fatty acids at different concentration. Real-time quantitative PCR and Western-blotting were used to detect the expressive of adiponectin mRNA and protein, moreover, relative mRNA expressive levesl of PPARγ2, SREBP-1c, C/EBPα, TNF-αand IL-6 were examined, respectively, in order to identify the regulatory of FFAs on adiponectin expression in matured adipocyte.1. After differentiation, the 3T3-L1 preadipocytes turned to be larger and round rather than of spindle shape, and contained large droplets of triglycerides. Over 90% of the preadipocytes appeared to be diferentiated into maturea dipocytes on day10. The oil Droplets in the cytoplasm were observed clearly under microscope. The differentiation were homogeneous and with high efficiency.2. all types of fatty acids inhibitted adiponectin mRNA expression in dose and time-dependent manner; moreover all the fatty acids significantly influence secretion of adiponectin protein.3. The fatty acid PA mainly through increased IL-6, TNF-αmRNA to inhibite the expression of adiponectin gene and its secretion. Polyunsaturated fatty acid AA, EPA and DHA affected adiponectin levels by suppressing the transcriptional activity of PPARγ2 and SREBP-1c.4. All types of fatty acids examined didn't affect adipoR1 mRNA expressive level. Under PA and OA treatment, the adipoR2 mRNA level decreased 28% and 20%, respectively; the rest of the fatty acids had no signifinant effect on adipoR2.