A Study Of The Embryonic Mouse Exerted Skin Nerve Endings Which To Meet The Amniotic Fluid

ObjectiveTo investigate the regular pattern of the development of embryonic mouse skin nerve endings at different stages and to see whether they are tend to contact with the amniotic fluid, to identify whether there are some relationships between such endings and the amniotic fluid. Then give an analysis of the role that any of the specific substances in the amniotic fluid play on the nerve endings development which innervated on the skin epidermis surface.MethodsTo get frozen sections of embryonic mouse from 12 to 17 days old, and then give a staining of neuron specific marker neurofilament with immunohistochemical technique. Then to observe the distribution of the nerve endings innervated on the skin surface with light microscope and take photos of such endings. Fluorogold was also injected into the amniotic fluid of the mother mouse at different periods of pregnancy to trace the origin of exserted nerve endings.Then frozen sections of Fluorogold successfully traced embryonic mouses were made to observe Fluorogold labeled neurons. ResultsThere are considerable nerve endings innervated on the surface of the embyonic mouse skin. It is already clearly to see the nerve endings toward the amniotic fluid when the Embryo is 12-day old and it is most evident of 15-day-old embryo. Fluorogold labelled neurons can be seen at the dorsal root ganglion (DRG), nerve root and the ventridorsal grey matter.ConclusionEmbryonic mouse skin innervations maybe exsert out of the skin surface and inserted into the amniotic fluid of the mother mouse. Such nerve endings may have some important connections with the amniotic fluid. It is very possible that amniotic fluid plays some important roles in the growth of embryonic mouse peripheral nerveous system, especially the skin nerve endings of the embryos. Another possibility is that the embryonic mouse skin nerve endings which extent to the amniotic fluid can identify the changes of the amniotic fluid, but the mechanisms how they react to each other is not clear now and need to be further studied. The relationship between amniotic fluid and the development of the skin nerve endings of the embryos also needs further experiments to verify.