Screening Signal Transduction-regulated Genes From Gene Chips In NPC And Then Making A Preliminary Identification Of The Candidate Gene

Objective:Nasopharyngeal carcinoma(NPC) is one of epithele origin malignant tumor, most of them are poorly differentiated squamous-cell carcinoma, and the cancer cell will reach the lymphadens of all over the body, and the death rate is very high.While the pathogenesy of NPC is unclear now. This study was to screen signal transduction -regulated genes from gene chips in NPC and then make a preliminary identification of the Candidate Gene.Methods:A preliminary selection of abnormal expression genes from the gene chips of the Shenzhen Weixin company which detected the seven nasopharyngeal carcinoma and one rhinopharyngitis. And then to integrate the data of signal transduction -regulated by Gene Ontology, screen signal transduction -regulated genes from gene chips in NPC from BioCarta,make a preliminary identification of the Candidate Gene by real-time quantitative PCR..Result:1.In the basic of gene chips, there are 871 high expression genes and 343 low expression genes in the nasopharyngeal carcinoma tissue.2.There are 27 differential expression relate gene of signal conducting.21 of them are high expressing and 7 are low express.They are NRG2,SCYA3,PDGFA,SCYA18,SCYB10,SCYA4,INHBA,SCYA2,SCYA13,EFNA1,SFRP1,IL1A,HDGF,DKK1,TNFSF7,ECGF1,TNFSF4,NRTN,GRN,S100A9,MIF,IGFBP5,GRO1,SDF1,IGF2,C3,PTN,SEL1L。 3.We accepted 7 signal transduction -regulated genes from BioCarta which could play important role in the development of NPC. And we got several signal pathways.4.We found that the two gene PDGFA and MIF high expressed in NPC,and that was according to the gene chips.Conclusion:1.We found a new way how to screen signal transduction-regulated genes from gene chips in NPC and then make a preliminary identification of the Candidate Gene.2.The differential expression relate gene PDGFA and MIF play an important role in the nasopharyngeal carcinoma's emerging. And our study can be helpful in the pathogenetic and the gene therapy of nasopharyngeal carcinoma.