The Investigation Of The Antitumor Effects Of Dioscin

With the previous researches,dioscin has been found to show strong immunoloregulative activity.However,dioscin whether or not has some antitumor effects is not known.To detail the effective mechanisms of dioscin,we investigated the in vitro experiment including MTT assay and wound healing assay.In our experiments, Lewis cells were exposed to dioscin.Cytotoxicity was determined in terms of cell growth inhibition(IC₅₀).The results of MTT assay showed that dioscin could inhibit proliferation of Lewis cancer cell line in different degree and it had dose-cytotoxicity relationship in a certain degree.The IC₅₀ was 3.85±0.11μg/mL.Migration inhibition was evaluated by wound healing assay in non-cytotoxic dose.The results demonstrated that dioscin exhibited remarkable cytotoxicity and caused typical apoptosis in a dose-dependent manner,whose concentration ranging from 0.2 to 1.0μg/mL.Dioscin was evaluated in vivo by its antitumor effect on Lewis inbred mice.In the tumor weight,spleen index,antitumor rate and numbers of mets comparison,the oral administration to mice of dioscin(60 mg/kg/d for 14 days) significantly inhibited tumor growth by 39.44%.The spleen index was 6.23mg/kg which was similar to the model group.It also could inhibit Lewis cell metastasis to lung and have no toxicity on main organs.In conclusion,dioscin exhibited strong antitumor and anti-metastasis in lung cancer mice.The study focused on the investigation of the antitumor effects of dioscin.The results of MTT assay showed that dioscin could inhibit proliferation of Lewis cell line in different concentration and it had dose-cytotoxicity relationship in a certain degree.The IC₅₀ was 3.85±0.11μg/mL.In addition,it exhibited anti-migration in the wound healing assay in different concentration ranging from 0.2 to 1.0μg/mLDioscin was evaluated in vivo by its antitumor effect on Lewis mice.The oral administration to mice of dioscin(60 mg/kg/d for 14 days) significantly inhibited tumor growth by 30.68%.It also could inhibit Lewis cell transfer to lung and has no toxicity on main organs.In conclusion,dioscin is a potent anticancer agent that inhibits the migration in murine lung adenocarcinoma,both in vitro and in vivo.Dioscin exhibited strong antitumor and anti-metastasis in lung cancer mice.