The Study Of Relative Risk Factors, Species Candida And Its Drug Susceptibility Test Of Oral Fungi Of Inpatients From The First Affilia Ted Hospital Of Kunming Medical College

Part1 Status of oral fungal infection and the relative risk factors of oral fungal infection.Objective To find out status of oral fungal infection and its relative risk factorsMetheds In this study 579 inpatients of 16 departments from the First Affiliated Hospital of Kunming Medical College were investigated,and their oral secretion samples and clinical information were collected, Identification of fungi was performed by direct microscopy, fungal culture.Results The experiment collected 579 cases of inpatients' samples from 16 departments of the First Affiliated Hospital ofKunming Medical College. From 579 samples,228 strains of fungi were isolated including 80 patients with Oral fungal infection (13.82%),and 148 patients with oral fungi- carrying (25.56%). Analysis of risk factors for oral fungal infection showed:patients who had long hospital stay had higher rate of oral fungal infection than those who did not (P<0.05), usage of antibiotics had higher rate of oral fungal infection than those who did not (P<0.05), the longer course of antibiotic usage had higher rate of oral fungal infection (P<0.05), combined or alternative usage of antibiotics had higher rate of oral fungal infection (P<0.05), Thoes who used glucocorticoid, especially betamethasone and dexamethasone had higher rate of oral fungal infection, large daily dosage and long treatment of glucocorticoid would increase the rate of oral fungal infection,(P<0.05),Those who stay in hospital for a long time after surgery got oral fungal infection easily(P<0.05). Analysis of risk factors for oral fungal carriers showed:1-10 years old and above 50 years age inpatients had higher risk of oral fungi- carrying (P<0.05). Inpatients who had respiratory diseases were more likely to be oral fungal carrier(P<0.05).Conclusion Unvaried logistic regression analysis showed that length of hospital stay, whether to use antibiotics, length of antibiotics treatment, combined or alternative antibiotics treatment, whether to use glucocorticoid, kinds of glucocorticoid, daily dosage of glucocorticoid treatment, length of glucocorticoid treatment, length of hospital stay after surgery were risk factors of oral fungal infections. Age and respiratory disease were risk factors of oral fungi-bearing. In clinical Practice,doctors should closely monitor the condition of patients who had risk factors mentioned before.Part2 The distribution and drug susceptibility spectrum of oral fungi in in patientsObjectic:To investigate the distribution and drug susceptibility spectrum of oral fungi in inpatients.Methods:The yeasts isolated from patients were cultured and purified routinely, then the species of isolates were indentified by CHROMagar culture media and API 20 C AUX. Sensitivity of the isolates to Fluconazole,Itraconazole, Voriconazole and Amphotericin B was detected with checkerboard micro dilution method based on M27-A2(2002) of NCCLS.Result:In this part 242 srains were separated and identified,Among them,the most common specie was candida albicans (174srains 71.90%).There were 68 strains non-candida albicans in all the isolates (28.10%). The differences ofratio of non-candida albicans of groups of age,antibiotics treatment and glucocorticoid treatment did not have statistical significance (P>0.05). Most isolates were sensitive to Fluconazole, Voriconazole and Amphotericin B. Non-candida albicans likely had lower sensitivity to Fluconazole compared With candida albicans,The differences were significant (P<0.05).Conclusion:Candida albicans was the most common specie in oral fungal infections and fungi-bearing. Most isolates were sensitive to Fluconazole, Voriconazole and Amphotericin B. Non-candida albicans likely had lower sensitivity to Fluconazole.