The Evaluation Of Serum Levels Of Folate, Homocysteine And MTHFRC677T Mutation In Women With Recurrent Spontaneous Abortion

Objective To investigate the effects of elevated homocystein levels in serum to the patients with recurrent pregnancy loss as well as the relationship of the level of the folate and MTHFRC677T mutation.Methods The MTHFR genotype was determined for 30 control healthy women and 49 unexplained recurrent early fetal loss patients by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)method.The concentration of serum homocystein and folate was determined.Results were analyzed with an x2 test for homogeneity. One way ANOVA and T test were used to compare serum homocystein levels between genotypes and groups,to determine the relationship between serum homocystein levels and folate,also Spearman correlation and odds ratio were used.Results Serum concentrations of homocystein showed significant differences between the two groups.The incidence rate of homozygotic TT and CT genotype in experimental group is 12.2%and 51%,as well as 6.7%and 23.3%in control group. There is an adverse relationship between the serum concentrations of folic acid and homocysteine. There was a significant difference in prevalences of the MTHFR genotypes (T/T, C/T and C/C)between the unexplained recurrent early fetal loss patients group (12.2%,51.0%and 36.8% respectively)and the healthy women group(P=0.016).Conclusion The serum concentrations of folic acid and MTHFRC677T are significantly altered in women with unexplained recurrent spontaneous abortions.The effect of folate deficiency might be amplified by MTHFRC677T mutation and might lead to hyperhomocysteinemia.