Methylation Analysis Of IL-10 Promoter In The Patients With Chronic Hepatitis B And Acute On Chronic Liver Failure

Objective:Detect the serum level and relativity of IL-10 and the methylation of its promoter, to investigate the effect and machanism of IL-10 the inflammatory reaction of chronic hepatitis B (CHB) and acute on chroinc hepatic failure patients.Methods:There are three groups of patients:the hepatic failure group, the CHB group, the healthy control group, with total of a 25 patients (22 men and 3 women),25 patients (19 men and 6 women),10 patients (5 men and 5 women), respectively.1,detect the serum level of IL-10 via ELISA in all patients, to analyze the difference among the three groups;2,detect the relativity of serum level of IL-10 in hepatic group with other related parameters as ALT, HBV-DNA, TBIL, PTA, MELD, etc, via correlation analysis, to investigate the relationship between level of IL-10 and degree of patient conditions;3,detect the methylation of IL-10 promoter via MSP in three groups, compared with the serum level of IL-10 to investigate the mechanism of IL-10 in hepatitis patients.Results:1,Both the hepatic failure group and the CHB group have significant increase in serum level of IL-10 compared with the control group (p<0.05); the serum level of IL-10 in CHB group is higher than the hepatic failure group, however without statistical significance (p>0.05); 2,the serum level of IL-10 in hepatic failure group has no significant relativity with level of ALT and HBV-DNA (r=-0.022, r=0.033, respectively; P>0.05); a positive correlation with level of TBIL (r=0.566, P<0.05); and negative correlations with level of PTA and MELD (r=-0.581, r=0.443, respectively; P<0.05);3,the distribution of the methylation of IL-10 promoter in hepatic failure group is significantly different from the other two; while no significant difference between the CHB group and the control group;4,the serum level of IL-10 has a significantly negetive correlation with its methylation (r=-0.878, P<0.05)conclusions:1,the serum level of IL-10 in hepatitis patients is significantly higher; the hepatic failure patients have a certain increase in IL-10 compared with CHB;2,The serum level of IL-10 in hepatic failure patients has a signifiant relativity with levels of TBIL, PTA and MELD, suggesting that the serum level of IL-10 is increasing with the degree of hepatic failure;3,The negetive correlation between level of IL-10 and methylation of IL-10 promoter suggests that the CpG methylation in promoter may be important in the machanism of gene inactivation.