The Empirical Study On The Expression Of Recombinant Plasmid PEGFP-N1-IGF-1 In The Osteoporosis Rat

BackgroudAs an important growth factor of promote synthesis and growth metabolism of bone, the decline of Serum IGF-1 levels has been proven to be an important reason leading to osteoporosis.Gene therapy for osteoporosis and other bone metabolic diseases has becoming a direction of the research, therefore, we used the recom-binant plasmid pEGFP-N1-IGF-1 in vivo transfection of osteoporosis rats to study the feasibility of improving their serum IGF-1 levels as well as bone quality in this experiment.ObjectiveTo study the expression as well as therapeutic effect of recombinant plasmid pEGFP-N1-IGF-1 in the osteoporosis rats in vivo, so as to make theoretical preparation for the treatment of osteoporosis using this recombinant plasmid.MethodsIn this study, a total of 30 normal female SD rats were divided randomly into three even groups.The rats in sham group was unovariectomized while in group pEGFP-N1 and pEGFP-N1-IGF-1 was ovariectomized. After 12 weeks, the three groups were given saline, pEGFP-N1 vector and recombinant plasmid pEGFP-N1-IGF-1 complex liposomes hydrodynamic injection via the tail vein in proper order. Fluorescence in vivo imaging and liver biopsy observations, serum concentrations of IGF-1 were determined at regular intervals. Also, bone mineral density and bone biomechanical parameters were measured.Results:1.After transfection, rats of group pEGFP-N1 and pEGFP-N1-IGF-1 could be seen noticeable fluorescence expression not only in the whole body but also liver biopsy, which was significantly different compared with the sham group.2. The expression level of IGF-1, lumbar bone mineral density and bone biomecha than nical parameters of group pEGFP-N1-IGF-1 were significantly better that of group pEGFP-N1 (P＜0.05), among them, the data of latter two index has no significant statistical difference with sham group (P＞0.05).ConclusionsRecombinant plasmid pEGFP-N1-IGF-1 can be successfully transfected and expressed IGF-1 protein in osteoporosis rats and formed a definite therapeutic effect, which can be a substantial foundation for further application of IGF-1 gene therapy to osteoporosis.At the same time, it is can be predicted that osteoporosis and ischemic necrosis of the local organization led by diabetes would become the indication for this gene transfer method.