| Objective: To evaluate the random flap in rats ET, TXB2 content of topical application of natural, recombinant hirudin on the blood supply of random flap.Methods: Transferring over 21 health Wistrar rats, weighing 250 ~ 300g, were divided randomly into 3 groups(7 in each group). A Group: Locally injected with natural hirudin; B Group: Locally injected with recombinant hirudin ; control group C: locally injected with isotonic Na chloride. A 10cm×3cm full thickness dorsal flap with the pedicle remaining attached at the posterior end was elevated in the rats. Set off directly after flap replanted, and after operation in a timely manner, after 3 days, 5 days after the flap remote from each group were given equal amount of the corresponding local subcutaneous injection of drugs. Regularly observed in each group of diet, mental, activity and skin color, edema and other conditions. Time after the operation, after 3 days, after 5 days and 7 days after tissue flap in center, made of tissue detection of endothelin (ET), thromboxane B2 (TXB2) content. 7d after the operation, the flap paddle survival of each group were measured planimetry to get the survival area and proportion. Then the rats were sacrificed and the survival tissue of the flap was sectioned amount of the inflammatory cell, the capillary, etc.. Results: Gross observation: three groups of rats, n = 7. A (natural hirudin group) an accidental death after the fifth day of anesthesia, and the remaining rats were no deaths, no postoperative flap infection. B (recombinant hirudin group) rats were not killed, an improper operation of surgery, postoperative flap infection and necrosis of large area (to remove), and the remaining flap without infection. C group had no deaths, no postoperative flap infection. A, B postoperative mental state better than the control group, consumption of time earlier than the control group. Immediately after the three groups no significant difference in skin appearance, after the first 3 days, there were three distal area of different color changes, skin flap necrosis area congestion regional boundaries and survival began. A group with no significant difference between group B, B group congestion area of more than A group (P> 0.05), the difference was not statistically significant. C group congestion is larger than A, B groups (P> 0.05), the difference was not statistically significant. 5 days after surgery, three groups of congestion distal necrosis area and regional boundaries survival than 3 days after surgery significantly to the proximal spread. A group B group and no significant difference, B group congestion area of more than A group (P> 0.05), the difference was not statistically significant; C group (control group) congestion is larger than A, B groups (P> 0.05), the difference was not statistically significant. 7 days after surgery, three groups of distal tip necrosis regional stability, survival and necrosis boundaries more clearly, A and B was no significant difference, B group congestion area of more than A group (P> 0.05), the difference was not statistically significant , C group necrotic area of more than A, B group (P <0.05), the difference was statistically significant. Histological observation: After an immediate, no significant difference between the three groups. After 7 days, A, B two skin structure is still clear, mild edema and subcutaneous microvascular more than C group. 7 days after the determination of flap survival: A group 7 days after flap survival rate (95.19±4.53)%, B group of flap survival rate (95.47±3.65)%, control group C group flap survival rate (87.59±3.34)%. A, B two sets of flap survival rate was significantly higher than C group, P <0.05, significant difference; A group of flap survival rate higher than the B group, P> 0.05, the difference was not statistically significant. Flap measurement of ET-1 levels: immediately after surgery, postoperative day 3 A, B, C three flap ET-1 showed no significant difference, P> 0.05, the difference was not statistically significant. After 5 days, A group of skin tissue ET-1 content was 157.49±42.03 pg / ml, B group was 170.07±35.67 pg / ml, control group C group was 218.64±43.77 pg / ml, control group flap ET-1 levels higher than A, B group, P <0.05, significant difference. A group of skin tissue ET-1 levels lower than the B group, P> 0.05, the difference was not statistically significant. 7 days after surgery, A group of skin tissue ET-1 content was 114.32±33.04 pg / ml, B group was 130.25±26.19 pg / ml, control group C group was 261.53±22.01 pg / ml, control group C group skin valve tissue ET-1 levels higher than A, B group, P <0.05, significant difference. A group of ET-1 levels lower than the B group, P> 0.05, the difference was not statistically significant. Flap TXB2, measured: after 3 days, 5 days, A group of flap TXB2, respectively, 60.36±47.03 pg / ml, 78.87±51.13 pg / ml, B group flap TXB2, respectively, 55.54±26.61 pg / ml, 61.02±25.28 pg / ml, control group C group flap TXB2 contents were 137.36±71.96 pg / ml, 140.13±43.77 pg / ml, control group, flap TXB2 were higher than A, B two flap TXB2 content, P <0.05, significant difference. A group of flap TXB2 was higher than B, P> 0.05, the difference was not statistically significant.Conclusion: Topical application of natural and recombinant hirudin in rats after transplantation of random flap flap in the ET, TXB2 decreased. Infer natural and recombinant hirudin on random flap transplantation may improve the role of venous congestion. Natural and recombinant hirudin on Comparison of venous congestion, no significant difference between the two. May be related to dose, time, concentration and other related factors. These and hirudin specific mechanisms to improve the venous congestion, need further study.
|
PDF Full Text Request