Effects Of Erythromycin On Cigarettes-induced Inflammatory Mediators In Human Macrophage

Objective: To study the effects of Erythromycin on cigarette smoke extract (CSE) induced interleukin 8 (IL-8) and nuclear factorκB (NF-κB) in human macrophages cells.Methods: U937 cells was cultured and induced by PMA(10ng/ml). Human macrophage growth curve was recorded by MTT and observed the effects of CSE (0.1%, 1%, 2.5%) and Erythromycin (0.1, 1, 10ug/ml). Macrophages were divided into control group, NF-κB inhibitor group, CSE group, and Erythromycin + CSE group. Control group was treated without anything. NF-κB inhibitor group was treated with CSE (1%) first, and then treated with NF-κB inhibitor (PDTC). CSE group was treated with CSE (1%). Erythromycin + CSE group was incubated with Erythromycin (1ug/ml) first, and then treated with CSE (1%). IL-8 levels were measured in culture supematants by ELISA. Activation of transcription factor NF-κB was evaluated by electrophoretic mobility shift assay (EMSA). The data was processed using SPSS 13.0.Results: Erythromycin (0.1, 1, 10ug/ml) and CSE (0.1%, 1%) have no effects on cell growth, but 2.5% CSE will damage cells. IL-8 levels had increased, after treated with CSE(1%)for 24 hours. Preincubation with Erythromycin (1ug/ml) for 24 or 48 hours significantly inhibit CSE (1%) induced increase of IL-8 levels. (24h, control group 103.52±3.33pg/ml, CSE group 264.93±14.88pg/ml, Erythromycin + CSE group 196.70±14.13pg/ml; 48h, control group 114.03±8.01pg/ml, CSE group 283.02±15.62pg/ml, Erythromycin + CSE group 214.17±13.71pg/ml; P<0.05). Activation of transcription factor NF-κB had increased, after treated with CSE (1%) for 24 hours. Preincubation with Erythromycin (1ug/ml) for 24 or 48 hours significantly inhibit CSE (1%) induced increase of activation of transcription factor NF-κB.Conclusion: CSE can make the transcription factor NF-κB activity increased, and then the cells increased the release of IL-8. Erythromycin can inhabit CSE-mediated IL-8 levels through down-regulation of NF-κB in human macrophages cells, and then influence the development of Inflammatory.