The Enhancement Of Sodium Salicylate On NMDA Receptors In Cochlear Spiral Ganglion Neurons Of Rats

Objective: investigating on the role of sodium salicylate on NMDA receptors in cochlear spiral ganglion neurons of rats to explore the ototoxicity mechanism of sodium salicylate on the ionic channel level.Method: The whole-cell patch clamp recording technique was used to record NMDA receptor currents in acutely isolated rat SGN , and the effect of sodium salicylate on NMDA receptor currents and pharmacological characteristics.Result: (1)No currents were recorded in application 0.1mM-10mM NMDA. (2) The inward currents were recorded giving sodium salicylate 5mM plus 0.1mM or 0.5mM NMDA, respectively.(n=5, 212.61±15.25pA,607.94±44.26 pA). APV eliminated completely the currents showing that the currents are from NMDA receptor.Conclusion: 1. NMDA can not individually elicit any recordable NMDA receptor current in SGN. 2. Sodium salicylate can enhance NMDA receptor current in SGN.