Research Of TIG2 Gene In Occurrence Of Acute Lymphocytic Leukemia Leukemia

Acute leukemia is the most common malignancy of children, including acute lymphoblastic leukemia (Acute Lymphocytic Leukemia, ALL) accounts for about 80%, currently the pathogenesis of ALL in children is not yet clear. With the application of molecular biology techniques, to find differences in gene expression profile of children with ALL had became to an important means for finding and reseasching mechanism of children ALL. A group of differentially expressed genes in children ALL leukemia were isolated successfully by suppression subtractive hybridization (suppression subtractive hybridization, SSH) technology, which TIG2 (Tazarotene-induced gene 2) gene expression was silencing or low. To further explore the pathogenesis of pediatric ALL, the eukaryotic expression vector pGL3-TIG2 were constructed successfully; ALL cells (c-ALL, pre-B ALL, B-ALL and T-ALL) were transfected cells, the gene expression of TIG2 were studied on leukemia cells. The results showed: Building a complete eukaryotic expression plasmid pGL3-TIG2 was constructed and proved by restriction analysis and DNA sequencing blast; ALL cells transfenctied by pGL3-TIG2 had expression of TIG2 observed by Western blotting; ALL cell proliferation was markedly inhibited by TIG2 transfected (P <0.01). Conclusion: The eukaryotic expression plasmid pGL3-TIG2 were constructed successfully; overexpression of TIG2 inhibited ALL cell proliferation and promoted apoptosis, the reseasch of TIG2 will provide a basis for the diagnosis and therapy of leukemia.