Effect Of Particle Size Of Hydroxyapatite On Growth Of Odontoblast MDPC-23

Objective:To observe the size-dependent effect of hydroxyapatite on the growth of odontoblast (MDPC-23).Methods:Three groups were designed according to the experimental materials used:1. nHAP group with 20nm Nano-hydroxyapatite,2. cHAP group with conventional particle size of the hydroxyapatite and 3. glass as control. Odontoblast MDPC-23 as the experimental cell was cultured in three groups. Morphology of cell were observed using Olympus inverted microscope, the growth of the cell in different groups were measured using Thiazolyl blue tetrazolium bromide test (MTT), and the growth curves of cell were drawn.Results:The MTT ratio of 20nm nHAP group was significant smaller than those in the cHAP group and the control (P<0.05); however, there was no significant difference after five-and seven-day's culture.Conclusion:20nm nHAP has no inhibitive effect on the growth of odontoblast MDPC-23, no different effect of particle size of hydroxyapatite on MDPC-23 growth was found.