| Gastric cancer is one of the most common cancer worldwide with high incidence and mortality.However,its clinical outcome still remains unsatisfactory.To explore the molecular mechanism of carcinogenesis and progression might improve its early detection,treatment and clinical outcome,and might also help to find new prognostic factors.MicroRNAs(miRNAs) are a newly discovered class of small noncoding RNAs encoded by the genomes of a wide range of multicellular organisms and negatively regulate gene expression at posttranscriptional level.It is predicted that each miRNA could regulate about 200 genes and the 1000 miRNAs in the human body may regulate the expression of up to 1/3 of human protein-coding genes,which implies the potential influence of miRNAs on almost every genetic pathway.Indeed,miRNAs have been shown to play crucial roles in many physiological and pathological processes,including cancer development and progression.They play an important role in the carcinogenesis. To date, a number of microRNAs have been found directly involved in the carcinogenesis of gastric cancer. Expression profiles of gastric cancer are closely related with staging, progress and prognosis. This experiment research stomach cancer and cancer are beside normally organize medium expression level and stomach cancer of miR-491 take place the contact of hair and exhibition and develop a new road for the stomach cancer prevention, clinical diagnosis and treatment.MethodsThe miR-491 express in fifty paired gastric cancer specimens and the corresponding adjacent normal mucosa was investigated by real time PCR. Turn to transfected into BGC823 cell with the mature body of miR-491 and anti-righteousness chain.Exploitation the MTT, flow cytometry and the transwell experiment, examine the change of its biology behavior, and make use of MTT, the flow type cell instrument and AO/EB to dye a method to observe it a growth to repress under the Efudex function with Apoptosis change. Adopt SPSS 13.0 covariance analysis software to carry on covariance to learn a processing, the basis different data type chooses a different covariance method. Statistical analysis was performed using the t-test and analysis of variance, P<0.05 was considered statistically significant.ResultmiR-491 expressions are obviously high in the stomach cancer organization in correspond the cancer normally organizes beside, and the stomach cancer malignant degree and stomach cancer cancer week organize medium miR-491 expressionses to measure into inverse ratio, the covariance learns a data manifestation p<0.05.Pass MTT, the flow type cell instrument, transwell small room etc. experiment data show, turn into microRNA 491 ex-bodies of BGC823 cell, grow speed to show Zhao to lower, expect the cell period to distribute obvious decrease in the s, change residence and encroach upon the ability also immediately descends and the cell Diao Wu comparison is obviously high at the matched control after the 5F function.The BGC823 turned into miR-491 anti-righteousness chains the cells then appear the above-mentioned contrary phenomenon.ConclusionExplicit stomach cancer cancer week organize medium miR-491 obviously low at the cancer beside normally organize medium miR-491 of expression, and the stomach cancer malignant degree and stomach cancer cancer week organize medium miR-491 expressions measure negative related.MiR-491 rise to repress a cell growth in the stomach cancer cell, obstruct cell division, lower cell to change residence and encroach upon an ability function.MiR-491 can influence a stomach cancer cell's sensitive degree for chemotherapy medicine, can show repress of the agree with stomach cancer cell of the Zhao exaltation fluorine urine Mi Ding and kill to harm a function in the high expression in the stomach cancer cell.MiR-491 low express the diagnosis of agree with stomach cancer to provide a certain help in the cancer the organization, may the further prevention and cure of also agree with stomach cancer provide new way of thinking.
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