The Detection And Clinical Signiflcance Of SFas And TNF-α In The Serums Of Patients With Chronic Obstructive Pulmonary Disease

objceitveTo investigate serum soluble Fas ligand (sFas) and Tumor necrosis factor-a (TNF-α) levels in the patients with COPD, and to examined correlations between serum biomarkers and the decline of lung function in COPD patients.MethodsSerum levels of sFas and TNF-αwere measured in 68 patients patients during acute episode and after disappearance of symptoms and 20 normal control by enzyme-linked immunosor-bent assay(ELISA). T-test was used to analysis the difference of every groups; the Linear correlation analysis were used to analysis the correlation between serum sFas, TNF-αand pulmonary function status.Results1.Serum levels of sFas in COPD acute phase, remission and the control group were (0.5534±0.1041) ng/ml,(0.4123±0.0550) ng/ml and (0.2902±0.0952) ng/ml, respectively. The serum levels of sFas in the patients with AECOPD showed increasing significantly compared with those in patients with stable episode and in normal group(t=4.472 P<0.01).2.Serum levels of TNF-a in COPD acute phase, remission and the control group were (49.36±11.31) ng/ml,(33.81±7.73) ng/ml and (16.03±5.22) ng/ml, respectively. The serum levels of TNF-a in the patients with AECOPD showed increasing significantly compared with those in patients with stable episode and in normal group(t=5.706 P<0.01).3. serum TNF-a and sFas levels in Severity AECOPD patients (55.25±20.20 ng/ml,0.5861±0.0988 ng/ml) were significantly higher than those in the middle grade AECOPD patients (43.50±13.30 ng/ml,0.4088±0.0517 ng/ml).4. serum TNF-αand sFas levels in COPD patients with smoking (38.66±17.74 ng/ml,0.4779±0.1837 ng/ml) were significantly higher than those in the nonsmoking controls(30.11±11.77 ng/ml,0.4088±0.0787 ng/ml,both P<0.05).5.Linear correlation analysis showed that the serum sFas and TNF-αin the patients with AECOPD was inversely correlated with Forced expiratory volume in one second (FEV11)(r=-0.256,-0.364, P<0.05).Conclusionsserum sFas and tumour necrosis factor-a (TNF-α) levels were increased in patients with COPD and was inversely correlated with FEV11, We conclude that increased serum sFas and TNF-αmay be associated with progression of COPD though inhibit the cell apoptosis.