| ObjectiveMicroglia cells are the most representative of the immune cells in the central nervous system. Since first described in 1891,the role of central nervous system of the microglia is more and more been attention. Microglia cells play a dual role of injury and repair in Parkinson's disease, Alzheimer's disease and brain inflammation, ischemia and many other nervous system diseases. Activated microglia in central nervous system innate immune responses has many functions, including immune inflammation, induction, phagocytosis, cytotoxicity and antigen presentation through regulation of T-lymphocyte responses. Microglia play a protective immune effects in addition to the central nervous system diseases caused by pathogens, in chronic or pathological activation time can cause tissue damage.Latest studies have shown that CD200-CD200R pathway is an important mechanism which can reduced microglia activation in the central nervous system. CD200 expression is in a wider scope, mainly in the nerve cells, thymus cells, B cells, T cells, vascular endothelial cells, etc.Its receptor CD200R, expresses mainly in monocyte-macrophage system (including macrophages, microglia cells, mast cells, etc.) and a bit of T cells,with its ligand CD200 very similar to the structure. CD200 and CD200R binding the system function which was triggered by intracellular signaling cascade, control and reduce the activation of monocyte-macrophage system.In this experiment we use BV2 microglia cells as an alternative,and PC 12 cells as replacement neurons, and we mainly study the expression of small glial cells the significant differences of CD200R in various immune conditions and to explore possible mechanism of the activated microglia cells damage the nervous. the main research microglia in different injury nerve environments whether the significant differences in expression of CD200R to explore the activated microglia cells and nerve interaction. MethodsIn the Logarithmic good growth phase of PC 12 cells treats with 500nM Rotenone (RT),after 24h changes conditione media(CM)respectively.BV2cells co-culiture with normal PC 12 cells,500nM RT treated PC 12 cells respectively and incubated with supernatant of normal and injury PC12 cells, normal 1640 medium culture BV2 cells as control.Two kinds of cells which were common cultured was intervaled by the transfer of sterile mesh (pore size 8μm in transwell).After co-culitured and incubated 24h cells were collected to test.We use MTT test cells viability, inverted fluorescent microscope observats cell CD200R expression ELISA quantitatively detects CD200R protein expression.Results1,The survival rate of BV2 cells incubated with supernatant of normal or damaged PC 12 cells is significantly lower than the control group (P<0.05), survival rate of BV2 cells cultured with PC12cells slightly higher than the control group but no significant differences (P> 0.05).2,when BV2 cell co-culture with 500nM RT injuryed PC 12 cells, CD200R expression compared with the control group were significantly increased (P<0.05), while incubated with impaired PC 12 supernatant,the expression of CD200R was significantly decreased compared with the control group(P<0.05).CD200R expression were slightly increased in the grroop of BV2 cells co-cultuered with normal PC12 cells,In the group of normal PC12 supernatant incubated with BV2 the expression of CD200R is slightly reduced, but they had no statistical meaning (P> 0.05).ConclusioThe supernatant of injury nerve cells can reduce the expression of microglia CD200R, while co-cultured with damage nerve cells that can enhance the expression of microglia CD200R...
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