Effect Of Topiramate On Expression Of Aquaporin 1 In Blood Plasma And Cerebral Tissue From Rat Model Of Migraine With Nitroglycerin

ObjectiveTo investigate the expression of aquaporin 1 (AQP1) in blood plasma and cerebral tissue from rat model of migraine with nitroglycerin and effect of topiramate (TPM) on the expression.Method50 SD rats were selected and randomly divided into 3 groups:control group:10 rats, modelset group:25 rats, treatment group:15rats. From the first day, rats of control group were given Normal Saline(2ml/kg) by hypodermic injection and given Normal Saline(2ml/kg) by lavage after 4 hour (to eliminate the effect to rats of hypodermic injection and lavage) from 1st day to 7th day; rats of modelset group were given nitroglycerin injection (10mg/kg) by hypodermic injection and were given Normal saline(2ml/kg) by lavage after 4 hour from 1st day to 7th day; rats of treatment group were given nitroglycerin injection (10mg/kg) by hypodermic injection and then were given topiramate (50mg/kg) by lavage after 4 hour from 1st day to 7th day. At 7th day, rats of each group were narcotized by 10%chloral hydrate at 4 hours after the last respective operation,then get blood and cerebral tissue, to measure concentration in blood and to observe histological change by light microscope and to measure values of average Integrated Optical Density (IOD) for AQP1 by immunohistochemical stains.Result1,survival of the rats:10 rats of control group survived,10 rats in 25 of modelset group survived,10 rats in 15 of treatment group survived, the difference of survival between modelset group and treatment group has statistical difference (P<0.05).2,results of AQP1 from ELISA:modelset group and treatment group show no statistical difference (P>0.05), control group and modelset group or treatment group both show statistical difference (P<0.05).3,results from HE stains:there were no abnormal changes obviously, and no obvious difference among groups.4,expression of AQP1 by immunohistochemical stains in cerebral tissue:average IOD value of control group show lower than that of modelset group or treatment group and both show statistical difference (P<0.05), modelset group show slightly stronger than treatment group,but show no statistical difference (P>0.05).ConclusionIn the attack of rat model of migraine with nitroglycerin, AQP1 may partake in the attack of migraine, but it cannot conclude that TPM maybe has effect on the expression of AQP1 as to be one of mechanisms of treatment for topiramate to migraine.