Construction And Potential Application Of Specific DNA Transduction Vector

At Persent, there is not a good measure in control and prevention of HIV infection. In order to control and prevention of HIV infection, the best effectively measure is developing a safe and effective vaccine to prevent HIV inefection.The purpose of this study is to build a new type of specific DNA transduction vector for the purpose of TG. Based on transcriptional activator protein (Tat), DNA sequence recognition and binding (GAL4/UAS), adding the appropriate Linker labeling and purification and identification of His tag, TG fragment has been cloned to vector pET-28a which come from Escherichia coli pastoris expression system for the expression plasmid pET-TG. G protein gene combined with the characteristics of UAS. The TG protein can be purified by in vitro and can be free associated with pVAX1-UAS-EGFP plasmid. The protein combining with plasmid transfect 293T cells. We can see that G and T of the corresponding gene of biological activity through the detection of green fluorescent protein EGFP.we obtain the escherichia coli expression system, we get the expression protein molecular weight is 21kDa. Plasmid 10μg can be seen the most capacity of binding protein of 16μg.Binding activity did not affect within 10-120min in time for TG.