| Human immunodeficiency virus (HIV) infection and AIDS continue to be a growing problem for the world's population. The need to develop a safe and efficacious vaccine against HIV is more pressing than ever.SIVmac239 Rhesus Macaque models are classical, which have been studied abroad for several years. In this study, according to the dosage ranges of HIV naturally sexual infection, two monkeys were rectally infected with three serial different doses of SIVmac239 virus. Meanwhile, the data of viremia and immune response were analyzed. The results show that both two monkeys were negative by RT-PCR and ELISA methods detection after serial challenge with 2×101 TCID50,2×102 TCID50 doses of virus. However, one monkey (M296) infected with 2×103 TCID50 for the third time established systemic infection and induced high level of humoral and cellular immune responses. In the study we explore the rectal infection mechanism in rhesus monkeys by mimicking sexual HIV infection, the dose-effect relationship on HIV sexual transmission was confirmed.A pathogenic R5 simian-human immunodeficiency virus (SHIV) encoding gene of the predominant prevalent HIV-1 B'/C Recombinant (CRF07_BC strain in China was highly desirable to study the role of HIV-1 envelopes in transmission and pathogenesis as well as to evaluate candidate AIDS vaccines in nonhuman primates. To this end, SHIVCHN19P4 also carrying CRF07_BC env, was adapted by serial passage in eight pig-tailed macaques. In this study, to evaluate the changes of virology and immunology in rhesus macaques infected with SHIVCHN19P4 and propagate SHIVCHN19P4 virus stock passaged in rhesus macaques, two juvenile rhesus macaques were infected intravenously. For serial passages,10 mL of whole blood from each of the P1 rhesus macaques at 60d was transfused intravenously into two naive rhesus macaques. Methods like flow cytometry, PCR, binding antibody and sequence alignment were analyzed the change features about those monkeys. Simultaneously, SHIVCHN19P4 virus stock was propagated by PBMCs co-culture. The Results show that all of four rhesus macaques established systematic infection, the infectivity of the virus is absolutely enhanced after passaging. Sequence analysis revealed SHIVCHN19P4 exerted adaptive changes. In the study our finding provided a firm basis for establishing of a SHIV infection model of Chinese rhesus macaques. This model would be very useful for HIV-1 subtype C vaccine and pathogenesis studies.To contain the pandemic of HIV/AIDS in china, both plasmids and non-replication competent Tiantan vaccinia (MVTT) containing gag, pol and env from SIVmac239 were constructed as experimental vaccines. To evaluate the immunogenicity and protective effect against homologous SIVmac239 challenge,4 Chinese-origin Rhesus Macaques were immunized with a MVTT-SIVgpe/SIVgpe plasmids boost strategy, while other 4 Chinese-origin Rhesus Macaques were immunized with a MVTT-s/pOPT9-S plasmids boost strategy as control group. Animals were followed prospectively for immune parameters. HIV-specific antibody titers and CTLs responses were determined with ELISA and ELISPOT based assay respectively. Compared to the control group, the numbers of vaccine-specific T cells raised by the MVTT-SIVgpe/SIVgpe plasmids vaccine, but HIV-specific antibody teters were low.A mouse-human chimeric monoclonal antibody-cMT807 can be selective elimination of CD8+lymphocytes in vivo. In this study,4 Chinese-origin Rhesus Macaques which had been infected SHIV virus were eliminated selectively CD8+ lymphocytes using cMT807. Methods like flow cytometry, PCR and ELISPOT were analyzed the change features about those monkeys. The results show that the viral load of the 4 monkeys was rebounded non-continuously and lowly after of CD8+lymphocytes elimination. In the study, a nonhuman primate model for the selective elimination of CD8+lymphocytes using a mouse-human chimeric monoclonal antibody. This model would be useful for exploring acute virus infection and pathogenesis.
|
PDF Full Text Request