Effect Of Quercetion On Testicle Injuries Induced By Diethylstilbestrol In Hamster

This study was conducted to investigate the protective effects of quercetin on DES-induced oxidative damage in cultured hamster spermatogenic cells. The cells were treated with different concentrations of DES, and the growth status were observed under inverted microscope. The viability of spermatogenic cells was detected by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT). The contents of superoxide dismutase (SOD) in supernatants and glutathione peroxidase (GSH-Px) in cells were detected with spectrophotography. In order to explore the protective effects of quercetin on spermatogenic cells and the possible mechanism, the expression of Caspase-3 were detected by western blotting method. The aim of the study was to look for the protective measures in male reproductive system induced by environmental estrogens.The experiment was divided into two parts. The purpose of part one was to investigate the effects of diethylstilbestrol on cultured hamster spermatogenic cells. The objective of part two was to explore the protective effects of quercetin on cell damage induced by DES.1 Effects of diethylstilbestrol on cultured hamster spermatogenic cellsThe spermatogenic cells of hamster were separated and cultured by combined enzymeatic method. The cells were treated with different concentrations of DES. The growing status of cultured cells was observed under the inverted microscope. The viability of spermatogenic cells were measured by MTT. The contents of SOD and MDA in supernatants and GSH-Px in cells were detected by spectrophotography. Results showed that the growth status of spermatogenic cells was well enough in control group and the survival rate is about 95%. While after exposure to DES, the viability of spermatogenic cells was decreased significantly compared with the control group (P<0.01). With the increasing dose of DES, the contents of SOD and GSH-Px all decreased, and the contents of MDA increased.2 Protective effects of quercetin on diethylstilbestrol induced oxidative damage in cultured hamster spermatogenic cellsThe spermatogenic cells were treated with DES and different concentrations of quercetin, and the growth status was observed under inverted microscope. The viability of spermatogenic cells was detected by MTT. The contents of SOD in supernatants and GSH-Px in cells were detected with spectrophotography. The results showed that quercetin significantly inhibited the DES-induced damage on spermatogenic cells, with the exception of the low-dose group in which no significant difference was observed. The cell survival rate increased significantly in the middle- and high-dose groups. The contents of SOD and GSH-Px were significantly elevated after medication with quercetin (P<0.01). The results of western blotting showed that Caspase-3 was expressed in each group. The expression of Caspase-3 was significantly enhanced in DES group compared with the control group (P<0.05).In present study, a significant reduction of cell viability was demonstrated after exposure to DES, accompanied with the decreasing integrity of spermatogenic cells. At the same time, MDA, an end product of lipid peroxidation elevated significantly, while the antioxidation defense system of intra-cellular was destroyed by the reduction of SOD and GSH-Px. However, after supplement with quercetin, the lipid peroxidation was decreased significantly, and the antioxidant defense system was markedly increased. The expression of Caspase-3 in testicular spermatogenic cells was significantly reduced in an dose dependent manner. It can be concluded that quercetin protects spermatogenic cells against DES-induced oxidative damage through increasing intracellular antioxidants and decreasing lipid peroxidation. Quercetin plays an important role in ameliorating reproductive damage induced by environmental oestrogens.