Human Cholangiocarcinoma Cell QBC₉₃₉ Multi-drug Resistance-induced Telomerase Activity In Different Stages Of The Study

Objective This issue for Long-term exposure chemotherapeutic drug-induced bile duct cells to produce drug resistance problems, The use of cholangiocarcinoma cells in vitro methods, Using molecular biology technology on human cholangiocarcinoma cell QBC939Chemotherapy-induced drug resistance, And to identify drug-resistant cholangiocarcinoma cells in varying degrees of drug sensitivity and the relationship between inhibition of telomerase activity.Methods In vitro using cell culture technology in human cholangiocarcinoma cell line QBC939 and expanded in vitro. And cholangiocarcinoma cell QBC939 carried out with different concentrations of doxorubicin resistance induction, Drug concentration in the target cells in the steady growth of the medium after passageUsing MTT method, Detection of cell sensitivity to different chemotherapeutic drugs to determine the extent of drug-resistant cells.The use of enzyme-linked immunosorbent assay method, Cell QBC939/ADM detect different levels of telomerase activity in drug-resistant. Analysis of cellular drug resistance and telomerase activity links.Results MMC concentration To 0.5ug/ml,1.0ug/ml,2.0ug/ml,4.0ug/ml four concentrations On QBC939 parental strain, induced by low-fold resistant strains, in times of drug induced strain, high-power induction of strains resistant to drug resistance were detected using MTT method, 490nm wavelength of light absorption measured value, Four concentration groups were analyzed statistically, Resistant strains induced low-fold compared with the group P<0.01, in times of resistant strains and the induced group P<0.05, resistance induced by high power lines with the group P<0.05, The group differences were statistically significant, The results suggest that mitomycin QBC939/ADM for a certain degree of drug resistance, From low-fold resistance to drug resistance induced by high-powered group showed an increasing trend of drug resistance.Concentration of 5-fluorouracil To 25ug/ml,50ug/ml, 100ug/ml,200ug/ml four concentrations On QBC939 parental strain, induced by low-fold resistant strains, in times of drug induced strain, high-power induction of strains resistant to drug resistance were detected using MTT method,490nm wavelength of light absorption measured value Four concentration groups were analyzed statistically, Resistant strains induced low-fold compared with the group P<0.01, Induced resistance in times of strain and the group P<0.05, high-power resistance induced by strains with the group P<0.05, The group differences were statistically significant, The results suggest that the 5-fluorouracil QBC939/ADM also has a certain degree of drug resistance, from low-fold resistance to the drug resistance induced by high-powered group also showed an increasing trend of drug resistance.Parental strains, low-fold induction of drug resistance strains, in times of strain induced resistance, high power resistant strains were induced by use of polymerase chain amplification and enzyme-linked immunosorbent assay method to detect telomerase activity, Determination of absorbance 450nm Department, As a result, a group comparison P<0.05 in each group were statistically significant differences. The results suggest that resistance to high power from low-fold induction group QBC939/ADM resistance tends to increase telomerase activity.Conclusion Adriamycin resistance in human cholangiocarcinoma cells after induction QBC939/ADM, pairs of mitomycin C,5-F both have different degrees of resistance; Human cholangiocarcinoma cell QBC939/ADM With the increase in drug resistance, telomerase activity increased.