Expression Of SRCAS1 In Aplastic Anemia Patients

Objective:Aplastic anemia (AA) in the pathogenesis is not clear, the present study suggests that dysfunction of the immune pathogenesis of its major. Immune dysfunction mainly in activated cytotoxic T cells and Thl cells increased, they secrete excessive negative regulator of hematopoietic factor, making way through the Fas apoptosis of hematopoietic cells Immunosuppressant by inhibiting cytotoxic T cells and activation of Thl cells treated AA. But the clinical findings are 20-30% AA patients immunosuppressive therapy ineffective. So, is there way except by methods other than Fas make excessive apoptosis of hematopoietic cells? Recent studies have found that macrophages can be expressed in SiSo cells secreted receptor binding cancer antigen (receptor-binding cancer antigen expressed on SiSo cells, RCAS1). Erythroid colony-forming cells on the expression of RCAS1 receptor and normal bone marrow cells of RCAS1 in combination, can induce apoptosis and regulate the proliferation and development of red blood cells. To measure sRCAS1 (the form of soluble of RCAS 1) of the bone marrow supernatant and bone marrow stromal cells supernatant of aplastic anemia patients, and the expression of RCAS 1 mRNA in bone marrow stromal cells of aplastic anemia; the expression of RCAS 1 in bone marrow cells. We analyzed the relationship between sRCAS1 of the bone marrow supernatant in AA patients and hemoglobin, platelet, leukocyte, reticulocyte. Explore the role of RCAS 1 In the pathogenesis of aplastic anemia.Methods:ELISA method for determining sRCAS1 of the bone marrow supernatant of 17cases aplastic anemia patients.Simple linear correlation analysis the concentration of RCAS1 that in bone marrow stromal cells supernatant of aplastic anemia with their RC.The expression of RCAS 1 in bone marrow cells of 12cases aplastic anemia patients was detected by Immunohistochemical.ELISA method for determining sRCAS1 bone marrow stromal cells supernatant of 17cases aplastic anemia patients.The expression of RCAS 1 mRNA in bone marrow stromal cells of 17cases aplastic anemia patients was detected by PCR.Results:The expression intensity of sRCAS1 in bone marrow supernatant of aplastic anemia patients(0.3709±0.1403)μg/l was significantly higher than that of normal controls (0.0874±0.1122)μg/l (P<0.05). The concentration of RCAS 1 that in bone marrow stromal cells supernatant of aplastic anemia were negatively correlated with their RC (20.04±14.62)×109/L, r=-0.941 (P<0.01).The expression intensity of RCAS1 in bone marrow cells of aplastic anemia patients (21.42±3.08)% was higher than that of normal controls (14.11±2.13)%(P<0.05).The expression intensity of sRCAS1 in bone marrow stromal cells supernatant of aplastic anemia patients (0.9989±0.0786)μg/l was higher than that of normal controls (0.8125±0.0489)μg/l (P<0.05).The expression intensity of RCAS1 in bone marrow stromal cells of aplastic anemia patients (0.61±0.48) was higher than that of normal controls(0.28±0.32) (P<0.05).Conclusion:Our results showed that the bone marrow supernatant and bone marrow stromal cell culture supernatant, and bone marrow cells and bone marrow stromal cells in the expression of RCAS1 in the AA group were higher, suggesting that RCAS1 may be involved in Aplastic Anemia pathogenesis. We found that RCAS1 of the bone marrow supernatant in AA patients has a negative correlation with the RC.It suggested that overexpression of RCAS1 and the incidence of aplastic anemia regulation of the hematopoietic. we conclude that AA patients with macrophages increased secretion of RCAS1 may be involved in the pathogenesis of aplastic anemia mechanism.