The Research Of Inducing Bone Marrow-derived Mesenchymal Stem Cells Marked By Superparamagnetic Iron Oxide(SPIO) To Differentiate Into Neural Cells In Vitro

Obiective To research whether there is any effect about it's cellular viability, proliferation and differentiation of neural cells after bone marrow mesenchymal stem cells(BMSCs)labeled with superparamagnetic iron oxide (SPIO) particles and to determine the best marking method and lay the foundation for stem cells in vivo tracing study.Methods Using superparamagnetic iron oxide labeling rats BMSCs, Prussian blue staining for identifying labeling index, TaiPan blue staining for detecting the cell vitality, MTT (tetrazolium blue) to detect proliferation activity of stem cells labeled. To induced stem cells labeled differentiating into nerve cells In vitro with 1mmol/LβMercaptoethanol (BME) and the Serum-free DMEM culture liquid, Then using immunohistochemistry to lay induced cells, using Prussian blue staining identifing the iron particles in nerve cells again.Results The stem cell marked rate with Prussian blue staining is 95%or more, the cell vitality rate with TaiPan blue staining is 97%, MTT method detect that it is not statistically significant for proliferation activity between labeled stem cells and not ones. Majority of bone marrow-derived mesenchymal stem cells differentiate into neuron-like cells After inducing by BME, immunohistochemistry was positive, using Prussian blue staining again shows iron particles is in the nerve cell cytoplasm.Conclusion There is no effect for survival, proliferation and differentiation to the nerve cells of stem cell after it is labled by superparamagnetic iron oxide.