The Experiment Study Of Neuroprotective On Vascular Dementia Rat Model By Using Edaravone

Objective:Through the establishment of vascular dementia (VaD) rat model, using the edaravone, to observe the impact of learning and memory ability, to detect MDA content and SOD activity in serum, immunohistochemistry was used to investigate choline acetyltransferase (Chat) and N-methyl D-aspartate receptor 2B (NR2B), to explore the functional mechanism and remedy effect of VaD with edaravone.Methods1-. Preparing VaD rat model. A "two-vessel occlusion+Nitroprusside buck" approach, namely as the artery with bilateral ischemia-reperfusion repeated twice, each time lOmin, at the same time, given 2.5mg/kg intraperitoneal injection of sodium nitroprusside before ischemia-reperfusion, to establish the rat model of VaD.2,The mechanism and efficacy on VaD rats with edaravone. Select the normal rats randomly; divide into sham operation group, model group, edaravone treatment group. For the sham operation group, detach the carotid artery, no ligation, no injection of nitroprusside, the rest of the groups used "two-vessel occlusion+Nitroprusside buck" approach, after modeling 7 days, edaravone treatment group were given edaravone of 3mg/kg by subcutaneous injection, one dose a day, last 20 d; the other groups were given the same dose of saline subcutaneously. At the 25th after modeling, each group was tested by Morris water maze,30 days after modeling all were executed.3,Taking the blood from abdominal aortric, using an improved thiobarbituric acid method to calculate malondialdehyde (MDA) content, using xanthine oxidase method to calculate the vitality of serum superoxide dismutase (SOD). At the same time, stripping the hippocampal of the rats, using immunohistochemical staining method to detect the expression of Chat and NR2B.Results:1,Morris water maze data suggest:the escape latency of the model group was significantly longer than the sham operation group, the difference was significant (P<0.01, P<0.05), the escape latency of treatment groups were less than the model group, with significant difference (P<0.05),2,The model group compared with sham operation group increased MDA content (P<0.05); Compared with model group, edaravone treament group decreased MDA content(P<0.05);In the three groups,there is no significant difference in increasing the of activity SOD. 3,The gray value of Chat and NR2B in the hippocampus of model group was highest, indicating its expression was the lowest(P＜0.01), the expression of Chat and NR2B in edaravone treament group was lower than in the model group (P<0.05).Conclusion:1,Vascular dementia rat model basically simulates the pathogenesis of VaD, and the whole experimental procedure is simple, less traumatic to the animals, higher survival rate, after modeling, learning and memory abilities declined, agreed the characteristics of VaD.2,There was a significant improved effect on VaD learning and memory impairment by using edaravone.3,Edaravone, through its role of anti-free radical to improve the symptoms of VaD.4,Edaravone, increasing the expression of Chat and NR2B, and then realize the protection of neurons.