The Experimental Study Of Influence On Synaptic Plasticity In Hyperbilirubinemia Born Rats Hippocampus

Objective To investigate the effect on rats'dentate gyrus synaptic plasticity of Hyperbilirubinemia,Revealed hyperbilirubinemia damage mechanism of learning and memory in rats ,For the prevention and treatment of children and provide experimental basis for learning and memory impairment.Methods①Modeling :1-week-old healthy SD rats were randomly divided into control group (CG) and test group 1 (TG1), test group 2 (TG2), test group 3 (TG3). The experimental rats were injected intraperitoneally with different concentrations of bilirubin solutions in order to establish animal models of hyperbilirubinemia;②Observation of rats in each group neurobehavioral activities and parts of rats in each group were randomly selected and using spectrophotometer and hippocampal brain tissue and serum bilirubin concentration, while light microscopic observation model of brain slices to determine whether to establish a successful;③Nerve Electrophysiology: model animals grown to 60 to 90 days, are used in place field potential recording method records hippocampal DG area, input / output curve (I / O curve), double-pulse response (PPF) and long-term potentiation (LTP), and to enhance the (DP) of the excitatory postsynaptic potential (EPSP) and population spikes (PS).④morphological detection: after the end of each recording experiment, Separation of animals with formalin-fixed hippocampus, sending light microscope observation of bilirubin deposition of particles in brain tissue. Results①By neurobehavioral activity, the brain specimens, and microscope general observation and bilirubin in serum and brain tissue concentration of indicators for identification model was successfully created.②and the control group, model group, I / O, a significant reduction in EPSP amplitude (P <0.05), PS amplitude also significantly lower (P <0.05);③model group, paired-pulse facilitation of the peak value of 146.16±16.97, less than the control group 195.89±12.01 (F = 12.12, p <0.01);④control group, LTP amplitude is 137.2±3% (EPSP), and 219.7±13.5% (PS), model group, compared with the control group the amplitude of LTP significantly reduced: 119.8±2.1% (EPSP), and 131.3±6.2% (PS) (EPSP: F = 80.811, p = 0.000 <0.01; PS: F = 34.2975, p = 0.000 <0.01);⑤model group compared with the control group was similar DP amplitude, the difference is not, no statistical significance.Conclusion Hyperbilirubinemia in neonatal rats can inhibit the basis of DG District granulosa cells synaptic transmission performance, thus affecting the granulosa cells simultaneously released, the damage paired-pulse facilitation and LTP in rat hippocampal DG area induced damage of the large offspring hippocampal synaptic plasticity...