The Effect Of Chronic Stress On Follicular Development And Mechanism Of Autocrine, Paracrine Regulatory Factors

Objective Observe the effects of chronic stress on follicular growth and the estrous and the effect on the follicle development of non-gonadotropin-dependented .Then checkout four paracrine-autocrine factors for RT-PCR,and see if they participate in the process of chronic stress on follicular growth and the development of a disincentive effect.Methods(1) Through the establishment of chronic stress model in mice ,and prepare HE sections to observe the impaction of the growth and development of ovarian follicles.(2) From the first day of chronic stress(to execute ended),mice were vaginal discharge for smear,Wright strain to observe the line of estrous cycle.(3) Added by exogenous gonadotropin to observe their effection of recovery on ovulation after chronic stress.(4) Using real-time PCR semi-quantitative experimental to determine the self-secreted paracrine factors which were selected. Results(1) Chronic stress extend and disorder the sexual cycle of mice .(2)Chronic stress Inhibite follicle maturation and ovulation occurring(3) Added exogenous gonadotropin can not make the follicular development and ovulation back to normal completely.(4) In the course of chronic stress, the secretion of paracrine factor GDF-9,BMP-15 from oocyte make a evidence reduction in the level of mRNA and SCF,bFGF from granulosa cells did not change.Conclusion Chronic stress can affect the estrous cycle in mice, affecting mouse follicle development and ovulation. Added exogenous gonadotropin, the chronic stress group significantly reduced the number of ovulation, HE staining showed: Chronic stress increased follicle atresia, interferes with follicular development and ovulation, even if add exogenous gonadotropin still can not make the early follicular development and ovulation back to normal, suggesting that the non-gonadotropin-class factors are involved in chronic stress interfere with follicular development and ovulation. RT-PCR results showed that after chronic stress, since the secretion of paracrine factor GDF-9,BMP-15 make a evidence reduction, SCF, bFGF did not change,suggest that chronic stress pass the way of down regulation the secretion of paracrine factors GDF-9,BMP-15, these factors may impact follicle development .