Angiotensinogen Gene M235T Polymorphism And Hypertrophic Cardiomyopathy

Objective: Hypertrophic cardiomyopathy is a primary autosomal dominant inheritant myocardial disorder characterized by asymmetric myocardial hypertrophy. Genetic heterogeneity and expressivity diffrence make the relationship between genotypes and phenotypes of hypertrophic cardiomyopathy complex. There are many studies about angiotensinogen gene (AGT) M235T polymorphism whether effect the relationship between the genotypes and phenotypes in hypertrophic cardiomyopathy patients worldwildly with inconsistent results, some studies found it maybe effect the relationship, while some studies found not. In a population of Anhui , we investigate the relationship between AGTM235T polymorphism and hypertrophic cardiomyopathy, especially the relationship between AGT M235T polymorphism and hypertrophic cardiomyopathy patients with left ventricular outflow tract obstruction which is a common and important phenotype. and discuss the factor which maybe effect the the expressivity diffrence in hypertrophic cardiomyopathy.Methods: A total of 94 hypertrophic cardiomyopathy patients in a population of Anhui were enrolled in the last five years diagnosed on the basis of echocardiographic criteria. And a total of 120 normal controls were enrolled at the same time without hypertrophic cardiomyopathy, and also without disorders that maybe assotionated with angiotensinogen gene M235T polymorphism such as hypertension, coronary atherosclerotic heart disease, and ischemic cerebrovascular disease. Polymerase chain reaction and sequencing directly were used to detect AGTM235T polymorphism of 94 hypertrophic cardiomyopathy patients and 120 normal controls. Data are expressed as means±standard deviation. Statistical analysis was performed with the SPSS 10.0 package. Chi-square test was used to test for genotype distribution and allele frequency and Hardy-Weinberg equilibrium of the polymorphisms. Multiple regression analysis was conducted for multiple genotypes between the two groups . A value of P<0.05 was considered significant.Results: The contribution of the genotype and the frequency allele of the AGTM235T polymorphism had no difference significantly in hypertrophic cardiomyopathy patient group and the control group(χ2= 0.29, 0.24, P >0.05). And those also had no difference significantly in hypertrophic cardiomyopathy subgroups regardless of there are left ventricular outflow tract obstruction or not(χ2=0.28, 0.15, P>0.05).Conclusions: AGTM235T polymorphism is not significantly associated with hypertrophic cardiomyopathy and left ventricular outflow tract obstruction. AGT M235T polymorphism maybe is not the modification gene which effect the expressivety diffrence in hypertrophic cardiomyopathy.