The Effect Of The Glucocorticoid Receptors Express In Cavia Cobaya Sauris Interna With Mth Of Different Density Injected Through The Cavum Tympani

Objective to investigate the effect of MTH of different density to the glucocorticoid receptor's express in cavia cobaya's auris interna injected through the cavum tympani. Then analyzing the possible mechanism of the administration through the cavum tympani.Methods MTH of three densities(32g/l,40g/l and 62.5g/l) injected through the cavum tympani, do the operation of decapitation to get the cochlea in 1h,1.5h and 2h after injection.The expression of glucocorticoid receptor in cavia cobaya's auris interna was detected by immunohistochemistry with monoclonal antibody of glucocorticoid receptor.Result glucocorticoid receptor got tinctured in each part of the cochlea after injected MTH of different density through the cavum tympani. The main espression part hanppened in ligament of cochlea, psalterial cord, acoustic organ and ganglion spirale. Compare with normal group, glucocorticoid receptors express got stronger in each part of the cochlea after injected MTH of different density through the cavum tympani. the intension of the receptors express gets stronger as the density of the MTH changes higher at the same time point. Under the condition of the same density, the strongest express appears at 1.5h after injection. That means the strongest express of the glucocorticoid receptor happened at 1.5h with injecting MTH of 62.5g/l. especially in spiral and psalterial cord.The difference of the receptors express has statistical significance.(p<0.05). theâ…¢wave's threshold of ABR had no statistically significan compared with the control group at 2h after injected three different density through the cavum tympani, P>0.05).Conclusion The expression of glucocorticoid receptor in cavia cobaya's auris interna can got stronger through injecting MTH in cavum tympani. And the intension of expression had relative with the time and density.