Effect On Intimal Hyperplasia Of Rabbits Jugular Vein Graft Treated With Glutaraldehyde

Objective To investigate the effect on intimal hyperplasia of rabits jugular vein graft treated with glutaraldehyde.Methods Sixty New Zealand white rabbits were randomly divided into experimental group and the control group, Before the anastomosis, experimental group vein was soaked in 1% glutaraldehyde solution for 10 minutes, and the control group vein in normal saline for 10 minutes.The vein graft harvested after 4 and 12 weeks.HE staining ,smooth muscle cellα-actin and proliferating cell nuclear antigen (PCNA) immunohistochemical staining were performed. Apoptosis was detected by TUNEL .Results 4 weeks and 12 weeks after grafting, The number of smooth muscle cell of the experimental group was significantly lower than that of the control group,also do intima thickness(0.016±0.004,0.031±0.008)mm,(0.025±0.005,0.093±0.024)mm(P<0.05). But two groups have no statistical difference in the rate of cell proliferation (P>0.05). The experimental group have higher apoptosis rate(3.3±0.7,6.7±1.5)% than the control group(1.2±0.4,1.8±0.5)% in 4 weeks and 12 weeks after grafting(P<0.05).Conclusion The rabbit jugular vein grafts pretreated with glutaraldehyde can significantly reduce the number of graft smooth muscle cells by increase the apoptosis rat and inhibit intimal hyperplasia, but do not inhibit smooth muscle cell proliferation rate.