| Object:Detected tight junction protein Claudin-1 (CLDN-1)in the tissues of phase I lung squamous cell carcinoma (SCC) patients,detected the expression of cytokeratins(Cytokeratin,CK)in lymph nodes with immunohistochemistry ,to known the relationship among the expression of CLDN-1 and lymph node micrometastasis,clinical and pathological factors as well as the survival time of patients , to provide a new theoretical basis for clinical treatment and prognosis of the Phase I Lung SCC patients.Methods:picked up 54 cases from First Affiliated Hospital of Guangxi Medical University of lung cancer, cancer adjacent tissues and lymph nodes with a complete follow-up data in 2002 to 2008.Examed the expression of CLDN-1 in lung cancer tissues and adjacent tissues, and CK in a total of 110 lymph nodes with immunohistochemistry S-P method. And detected 20 cases of inflammatory pseudotumor patients with the corresponding method. SPSS statistical software 17.0 were used for data analysis and P <0.05 as significant level.Results:(1) The expression rate of CLDN-1 were(+)21.37%,(++)40.74%,(+++)38.89%;in adjacent noncancerous were (+)83.33%,(++)16.67%,(+++)0;in flammatory pseudotumor were(+)75%,(++)25%,(+++)0.The difference between tumor tissue and adjacent noncancerous was statistically significant (P = 0.000), between tumor tissue and normal tissue was statistically significant ( P=0.000), between normal tissue and adjacent tissue was not statistically significant (P=0.506).(2)The difference of expression rate of CLDN-1 among the well-differentiated tumor tissue ,differentiated tissue and poorly differentiated were statistically significant (P=0.015),and the expression rate was not related with age, gender, tumor size and other factors.(3)In tumor patients with negative lymph nodes pathological examination, 15 were detected lymph node micro-metastasis, the detection rate of lymph node micrometastases was 27.78% (15/54), in flammatory pseudotumor were not detected micrometastases (4)The expression rate of CLDN-1 in lymph node micrometastasis patients were different from no lymph node micrometastasis , difference was statistically significant (P=0.000).(5)The expression of CLDN-1 was positive correlative with CK expression in lymph node (P=0.003).(6)The optical density value of CLDN-1 in tumor tissues was 0.434±0.0824, in adjacent tissues was 0.304±0.0279, in normal tissues was 0.308±0.0312.(7)K-M showed the prognosis of patients is correlated with histological grade,the expression of CLDN-1, lymph node micrometastasis.Log-Rank test suggested that the survival rate of patients with poorly differentiated is significantly lower than high differentiation,(P=0.000), CLDN-1 (+++) expression of patient survival rate was significantly lower than (+),(++) patients(P=0.003), survival rates of lymph node micrometastases significantly lower than those without metastasis,(P=0.000).Cox multivariate regression model showed that lymph node micrometastases,and CLDN-1 expression were the independent risk factors in lung SCC patients. Conclusion:(2) The expression rate of CLDN-1 in the phase I scc patients in tumor tissue was significantly higher than the adjacent tissue and normal lung tissue,its expression rate was no correlated with tumor size, age, gender,andcorrelated with the patients'histological grade, lymph nodes micrometastasis.The median survival time of high expression rate patients was shorter than low expression rate patients (P <0.05), CLDN-1 expression intensity could be increase the risk to reduce the survival time of patients. Lymph nodes micrometastases and CLDN-1 expression were the independent prognosis in patients. Therefore, combined detection of CLDN-1, lymph node CK expression can be used as an assessment of the biological behavior of lung cancer,forecasts transfer,guide treatment and prognosis.
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