Clinical Significance Of The Changes Of Serum TNF-α,IFN-γ,MMP-9,oxLDL Levels In Patients With Acute Coronary Syndrome

Background The activity of the inflammatory in Atherosclerotic (AS) plaque promote the progress of plaque unstable, thrombosis and acute coronary events. Various inflammatory factors, including TNF-α,IFN-γ, MMP9,oxLDL, play complex roles in AS,occurrence and development. Combination of two or more inflammation can improve application value about the diagnosis and treatment in coronary heart disease (CHD).Objective To investigate Clinical significance of the changes of serum TNF-α,IFN-γ,MMP-9,oxLDL levels in patients with acute coronary syndrome (ACS).Object of study Selected 63 patients who had been done coronary angiography from June 2009 to January 2010 in our hospital .There are 37 males and 26 females, They were been divided into 3 groups: acute myocardial infarction (AMI) group, unstable angina group and the normal control group.Experimental group According to the results of coronary angiography the patients were divided into 3 groups: acute myocardial infarction (AMI) group, unstable angina group and the normal control group. There are 27 cases in AMI group, 17 males and 10 females, mean age (62.5±11.4) years. Selection criteria: According to clinical symptoms, ECG changes ,myocardial enzyme increases,coronary angiography,all patients were Q-wave myocardial infarction, and the incidence in the 3h ~ 24h. There are 20 patients in UAP group, 13 males and 7 females, mean age (64.4±9.1) years of age. Selection criteria: Overworked deterioration of angina and (or) resting angina, and at least onseted once severe angina in 48h, electrocardiographic shows ischemic changes, myocardial enzymes normal, coronary angiography showed at least a coronary artery stenosis degree > 70%. The normal control group,there are 22 cases, 12 males and 10 females, mean age (60.8±9.4) years of age. Selection criteria: chest pain, ECG and myocardial enzymes normal, coronary angiography showed coronary artery no stenosis.Specimen AMI and UAP patients,blood were taken 5ml immediately after admission,the normal control group blood were taken 5ml in the next morning . The blood serum was obtained after centrifugated on the same day and placed in -20℃in refrigerator to preserve. Plasma concentrations of TNF-α, IFN-γ, oxLDL, MMP-9 were measured in a day.Experimental methods Using enzyme-linked immunosorbent test (ELISA) test ,kit were bought from Adlitteram Dignostic Laboratoris,Inc in USA. The operating instructions strictly according to the kit.To read each hole OD value through the length 450nm microplate . The sample values were taken into four parameters OD fitting formulae, calculated samples density.Statistical Methods Use SPSS14.0 statistical software applications to analyze, measurement data using xˉ±s, measurement data use t-test, count data usingχ2 test , P <0.05 for significant difference. Many factors applicate Logistic model do correlation analysis.Results:1. The plasma level of TNF-αin AMI group and UAP group, no statistical significance (P>0.05), AMI group compared with the control group the serum levels of TNF-αincreased significantly (P<0.05), UAP group compared with the control group the serum levels of TNF-αincreased significantly (P<0.05).2. The plasma level IFN-γAMI group compared with UAP group ,no statistical significance(P>0.05), AMI group compared with the control group the serum levels of IFN-γincreased significantly(P<0.05),UAP group compared with the control group the serum levels of IFN-γincreased significantly(P<0.05).3. MMP-9 plasma levels AMI group compared with UAP group the serum levels of MMP-9 increased significantly (P<0.05), AMI group compared with the control group the serum levels of MMP-9 increased significantly (P<0.05), UAP group compared with the control group the serum levels of MMP-9 increased significantly (P<0.05).4. The plasma levels of oxLDL AMI group compared with UAP group, no statistical significance (P>0.05). AMI group compared with the control group the serum levels of oxLDL increased significantly (P<0.05). UAP group compared with the control group the serum levels of oxLDL increased significantly (P<0.05).Conclusion:1.The plasma levels of TNF-α,IFN-γ,MMP-9 and oxLDL in patients with acute coronary syndrome are significant positive correlation.2.The plasma levels of TNF-α,IFN-γ,MMP-9 and oxLDL can be used to determine the nature of coronary atherosclerotic plaques.3.The plasma levels of MMP-9 increased hints plaques instability, Also can be used as an important biochemical monitoring index about the risk stratification of coronary artery disease.