Biological Activity And Blood Compatibility Of Metal Surface Modification

To reduce restenosis rate after the current metal coronary artery stent implanting, the paper trys to fabricate (Polyethylenimine(PEI)/Hyaluronan(HA))n, (Polyethylenimine(PEI)/ Heparin(HE))n, Polylactide glycolide(PLGA)/(Hyaluronan(HA)/Chitosan(CS))n and PLGA/ (HA/CS)n/CD34 bioactive coating on biomedical 316L stainless steel(316LSS) surface. The distribution and properties of bioactive coating is analyzed by using Contact angle measurement, scanning electron microscope(SEM), Fluorescence microscope and UV-visible spectrophotometer. Through the study the following major conclusions can be drawn:1. (PEI/HA)n bioactive coating is fabricated on biomedical 316LSS surface via electrostatic self-assembly. The hydrophilic coating is tested by contact angle. It is proved that the hydrophilic properties is improved by the (PEI/HA)n coating. In the platelet adhesion experiment, the blood compatibility and anticoagulant properties of 316LSS is improved.2. (PEI/HE)n bioactive coating is fabricated on biomedical 316LSS surface via electrostatic self-assembly. The heparin coating is tested by contact angle. It is proved that the hydrophilic properties is improved by the (PEI/HE)n coating. In the platelet adhesion experiment, the anticoagulant properties of 316LSS is improved. In dynamic blood clotting test, the changes is more slowly in dynamic lotting time curves and the clotting time is longer. It shows that blood compatibility is improved.3. PLGA(HA/CS)n bioactive coating is fabricated on biomedical 316LSS surface via electrostatic self-assembly. The hydrophilic properties of pretreated 316LSS by PLGA is improved by the coating. With the increasing number of the coating, the hydrophilic properties is improved. The erosion-resistant performance of the coating is studied in Kokubo PBS. In the platelet adhesion experiment, the blood compatibility and anticoagulant properties is improved. The anticoagulant properties of the coating by low concentration solution is better than high concentration.4. PLGA(HA/CS)n/CD34 bioactive coating is fabricated on biomedical 316LSS surface via electrostatic self-assembly. The hydrophilic properties of pretreated 316LSS by PLGA is improved by the coating. The erosion-resistant performance of the coating is studied in Kokubo PBS. The results show that the antibody coating has good adhesion. In the platelet adhesion experiment and dynamic blood clotting test, the antibody coating has good blood compatibility and achieved in loading antibody on the metal stent. It improved the restenosis capacity of anti-drug-eluting stent. It provides the theoretical and practical significance in improving the rapid endothelialization.