A New Method To Determine Parameters Of Interreaction Between Proteins And Drugs

The research of interreaction between proteins and drugs is always one of the hot spot in analytical chemistry and biological chemistry. The stratography has done significant contribution to the interreaction between proteins and drugs.The construction of theoretical model of interreaction between them is very impotant in spreading this method, and also is one of the science questions which should to be solved immediately. This article investigates in constructing and verificating of the theoretical model of interreaction between proteins and drugs by means of two parameters, injected amount of solutes and capacity factor. This article has three chapters to introduce all of the work, the contribution of the author as follows:1 This article provides one theoretical equation to describe the injected volume of solute (nb) and capacity factor (k') in the chromatography as the main parameters on the basis of the relationship between the solute and stationary phase. This equation can be used to determine the association constants (K) and binding sites (nα) on the stationary phase between the solute and stationary phase and to provide a theoretical basis of interaction between drugs and proteins.2 The bovine serum albumin (BSA) immobilized on the surface of macro-pore silica to prepare the BSA affinity chromatographic column was used to investigate the interaction between three kinds of drugs, omeprazole, propranolol, promethazine and BSA. The theoretical equation derivation in this article was used to deal with the datas. The results showed that the association constants of drugs and BSA were 3.83×104 L/mol,1.39×104 L/mol,1.24x×104 L/mol, and binding sites of them were 9.67×10-9 mol,2.77×10-8 mol, 8.05×10-8 mol, respectively. The values of K and nαwere confirmed to the results from classical method, such as fluorescence spectroscopy method and radioimmunoassay ligand method in the literatures, indicating that the proposed method can be used to investigate the interaction between ligands and functional proteins.2 Based on the researchs of interreaction of the BSA and drugs, immobilized receptor chromatography was used to investigate the interactions between the corresponding drugs and immobilizedα1A-adrenergic receptor (α1A-AR) andβ2-adrenergic receptor (β2-AR). The association constants of five kinds of drugs, berberine, phentolamine, terazosin, adnephrin, and metaramino withα1A-AR were 5.71×103 L/mol,3.37×103 L/mol,5.22×103 L/mol, 1.32×103 L/mol and 9.2×102 L/mol, and the binding sites onα1A-AR-bond medium were 2.22×10-7 mol, 1.01×10-7 mol,5.0×10-8 mol,4.2×10-8 mol and 6.4×10-8 mol, respectively. The association constants of five kinds of drugs, isoprenaline, salbutamol, noradrenaline, metoprolol and atenolol withβ2-AR were 1.66×103 L/mol,4.7×102 L/mol, 1.11×103 L/mol, 9.5×102 L/mol and 4.3×102 L/mol, and the binding sites onβ2-AR-bond medium were 1.49×10-7 mol,3.04×10-7 mol,1.31×10-7 mol,1.8×10-7 mol and 9.6×10-8 mol, respectively.