| Objective To study the systemic distribution of bone marrow derived mesenchymal stem cells (MSCs)in chronic myocardial infarction SD rats 24 hours,2 weeks and 1 month after cell intravenous implantation.Methods MSCs from male Sprague Dawley(SD) rats were cultured for transplantation. Three weeks after induction of myocardial infarction, 18 female SD rats were randomized into 2 groups. MSCs (5×106,300μl) were injected into the femoral vein of experimental group (n=9), meanwile phosphate buffered saline (PBS) of equal volume were injected into the femoral vein of control group (n=9). Another 9 female SD rats that underwent thoracotomy only were involved as sham operation group, and MSCs (5×106,300μl) were injected as the same way. The systemic distribution of MSCs were evaluated through Real-time Polymerase Chain Reaction (RT-PCR) at time points of 24 hours,2 weeks and 1 month after injection.Results 24 hours after MSCs injection intravenously, cells mainly concentrated in the lung in experimental group and sham group.The cell number was 2584269±647796 and 2901384±784872 respectively, and only few cells concentrated in the heart, spleen and liver.2 weeks after transplantation, there were few cells in all organs including the lung. Obvious cell loss was noted in all organs 1 month after transplantation.Conclusion For chronic myocardial infarction, most MSCs were early trapped in the lung after intravenous transplantation, however, substantial number of cells lost with prolonged time span. Only a small fraction of cells concentrated in heart, spleen, and liver.
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