Expression Of SM22a, A-SMA And OPN In Human's Thoracic Aorta Dissection And Change Of Vascular Smooth Muscle Cells' Phenotype

Objective:To explore the expression of SM22α,α-SMA and OPN in human's thoracic aorta dissection(TAD),and also the change of vascular smooth muscle cells'(VSMC) phenotype in dissected artery;to investigate the role of the VSMC's phenotype transformation in the disease of TAD.Methods:Tissues of dissected artery walls and normal artery walls were collected,protein was extraced from tissues which were also made of slices. Western Blotting study was performed to detected the expression of SM22α,α-SMA and OPN in these two groups while immunohistochemical study was performed to investigate the expression and location of them in slices.Results:SM22αandα-SMA expression was apparently downregulated in TAD group that was mostly happened in VSMC of artery media wall,especially in the tear point where "empty zone" was present.OPN expression was not significantly different between these two groups,but was with increasing tendency in TAD.In tissue slices,OPN was barely expressed in the normal artery walls while it was apparently found in TAD group,mainly occurred in VSMC of media walls.Hence, proliferated phenotype was predominated among VSMCs of the media in TAD group.Conclusion:VSMC phenotype was very different in two groups and proliferated phenotype was primary in TAD group.Therefore,the madia walls character was changed by VSMC's phenotype switched to the proliferated.Then,artery walls'elasticity became so poor that the media was tore through blood impluse,which eventually led to aorta dissection.