| Objective Compare the levels of Annexinâ…¡and t-PA in patients with systemic lupus erythematosus, diabetic nephropathy, chronic glomerulonephritis and in normal control group, to explore the significance of the Annexinâ…¡in systemic lupus erythematosus.Methods From January to October 2009, 35 cases of patients with systemic lupus erythematosus, 10 cases of patients with diabetic nephropathy, 10 cases of patients with chronic glomerulonephritis who were hospitalized in the nephrology department of the First Affiliated Hospital of Suzhou University were enrolled in this study, 20 cases of healthy controls were also enrolled. Circulating of Annexinâ…¡i n white blood cell was detected by flow cytometry; The level of plasma t-PA was measured by the enzyme-linked immunosorbent assay(ELISA); The blood pressure and other subjects were measured also; Fasting venous blood samples were extracted to detect hemoglobin, serum creatinine, blood cholesterol, blood glucose, prothrombin time, activated partial thromboplastin time, D-dimer; Urinary protein was detected by radioimmunoassay.Results1 Compare with healthy control cases, the level of Annexinâ…¡in white blood cell in SLE patients was significantly lower (P<0.01), the plasma level of t-PA was significantly higher (P<0.01); the level of Annexinâ…¡in diabetic nephropathy patients was also significantly lower than that of in control (P<0.05), but the level of t-PA had no significant difference with that of in control (P>0.05); the levels of Annexinâ…¡and t-PA in chronic glomerulonephritis patients had no significant difference (P>0.05).2 Compare with SLE group, the level of Annexinâ…¡in Diabetic Nephropathy patients had no significant difference (P>0.05), the plasma level of t-PA was significantly lower (P<0.01).3 In SLE group, the level of Annexinâ…¡in Lupus Nephritis patients was lower than that of in without Lupus Nephtitis patients, but it had no significant difference(P>0.05), the level of plasma t-PA was also higher , but with no significant difference either(P>0.05). In the LN patients who did the renal biopsy, the level of plama t-PA inâ…£LN was the highest in all LN patients(P<0.05), the level of Annexinâ…¡was decreased, but with no significant difference(P>0.05). The level of Annexinâ…¡of LN patients whom were more active decreased more than that of whom were less active(P<0.05), the level of plasma t-PA had no significant difference(P>0.05).4 The level of D-dimer in SLE patients was significant higher than healthy control(P<0.01), the levels of PT, APTT, Fbg had no significant difference(P>0.05).5 As for Annexinâ…¡, a positive correlation was found with serum albumin(r=0.439,p<0.01), negtive correlation was found with urine protein/urine creatinine(r=-0.382,p<0.05), SLEDAI(r=-0.417,p<0.05), D-dimer(r=-0.336,p<0.05), no correlation was found among 24 hours proteinuria(r=-0.319,p>0.05), total cholesterol(r=-0.230,p>0.05), hemoglobin(r=-0.005,p>0.05), creatinine(r=-0.204,p>0.05). As for t-PA, a positive correlation was found with glucose(r=0.602, p<0.01), ESR (r=0.53,p<0.01), IgG (r=0.468,p<0.01). No correlation was found between the t-PA and Annexinâ…¡(r=-0.014,P>0.05).Conclusion1 Patients with systemic lupus erythematosus have hypercoagulability and secondary hyperfibrinolysis states, the abnormal of coagulation and fibrinolytic systems are participate in the pathophysiological process of the occurrence and development of systemic lupus erythematosus.2 The decrease of Annexinâ…¡is one of the important reasons for hypercoagulability and thrombosis-prone in patients with systemic lupus erythematosus, and it can become one of the indicators of reflecting the disease's activity. 3 As a new marker, Annexinâ…¡can be used as a reflection of the abnormal of the coagulation and fibrinolytic systems, and it can be used as a good indicator of reflecting prothrombotic state in SLE patients, it can be helpful to judging the activity and the therapeutic effect of the disease.
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