Autologous Osteogenic Effect Of Embryonic Stem Cells Induced By Embryonic Stem Cells

Objective:To explore how endothelial cells (Ec) induced by embryonic stem cells (embryonic stem cells, ESCs) affect autologous embryonic stem cells when ESCs Transulate into OsteoblasMethod:Extract mouse embryonic stem cells and embryonic fibroblast,then induce embryonic stem cells to Ec. Cells were divided into four groups Group A embryonic stem cells Group B Induce embryonic stem cells Group C Induce endothelial cells GroupD Induce endothelial cells and embryonic stem cells.From Stem cell morphology, Immunofluorescence,Cell value and ALP,then Observe how endothelial cells affect embryonic stem cells when it induced Osteoblast from the enzymeResult:By immunofluorescence it is confirmed that the cell of C group was induced EC D Group:from MTT detection Cell proliferation in each group have no significant difference in meaning.(p>0.05)from Alkaline Phosphatase the Transformation of group D is better than the other groups.(p<0.05)Conclusion:endothelial cells induced by Embryonic stem cells into have a clear role when the Embryonic stem cells Transulate into Osteoblast...