Objective: Investigate meiotic abnormalities in spermatocytes of infertile men and discuss more Cytogenetics genetics mechanism in male infertility. Method: Using the spreading technique and immunofluorescent staining to prepare synaptonemal complexes from spermatocytes and antibodies against SCP3 , MLH1, and CREST to display lateral elements of the SC, recombination sites and centromeres. Results: 42% of the patients had a complete absence of meiotic cells.Compared with controls, these patients had significantly more cells in leptotene and zygotene, less cells in pachytene(P<0.05),and these patients had Significantly reduced recombination, increased frequency of achiasmate autosome bivalents, and a decrease frequency of sex univalents in pachytene(P<0.05).Meanwhile, a higher frequency of cells with spli(tunpaired chromosome regions )and achiasmate bivalents was found in these patients compared with the control group(P<0.05). Conclusion(s): Defects in chromosome synapsis and decreased recombination during meiotic prophase may be a important factor in spermatogenesis obstruction .
|