Effect Of Autologous Bone Marrow Stromal Cells Transduced With Osteoprotegerin Gene On The Periodontal Bone Regeneration In Beagle Dog Periodontal Window Defects

Objiective The purpose of this investigation was to evaluate the effect of the OPG gene-modified autologous bone marrow stromal cells(BMSCsOPG) combined with guided tissue regeneration on bone regeneration of periodontal window defect.Method pSecTag/2B-OPG was transduced into BMSCs by lipofectamine 2000.The expression of OPG protein in the BMSCs was detected by immunocytochemistry and western-blotting. Scanning electron microscopy (SEM) was used to observe the morphology and proliferation of the BMSCsOPG on the surface of the poly (lactide-co-glycolide) (PLGA). The periodontal window defects(4mm×4mm×3mm) were surgically created in the buccal aspect of the mandibular premolar (P2,P3,P4), and were randomly assigned to receive BMSCsOPG-PLGA(cells/material/OPG), BMSCs-PLGA(cells/material),PLGA(material),or root planing only (negative control). The animals were euthanized at 6 weeks post surgery for histological analysis. Histological measurements were performed with Image-Pro-Express system. All data were statistically analyzed using the Mann-Whitney U test.Results Six weeks after operation,the alveolar ridge regeneration amounted to a repair height of 3.82mm±0.45mm,1.12mm±0.12mm,0.67mm±0.14mm,0.33mm±0.09 mm and reached 52.88%,25.23%,16.42% and 7.80% of the original height in the experimental group,cells/material group, material group and negative control group respectively. Results showed that the height of new alveolar bone and cementum and the formation of new connective tissue were significantly greater in the experimental group than in the control groups (p<0.05) after 6 weeks, whereas there was no significantly difference in the material control and negative control (p>0.05). These findings suggest that delivery of BMSCsOPG-PLGA may be a viable approach to promote the bone regeneration of periodontal bone defects.