HIF-1α Gene C1772T/G1790A Single Nucleotide Polymorphism And Hypoxic-ischemic Encephalopathy And Cerebral Palsy Caused By Hypoxic-ischemic Encephalopathy Correlational Study

Objective To study hypoxic-ischemic encephalopathy/ cerebral palsy caused by the hypoxic-ischemic brain disease and HIF-1αgene C1772T/G1790A the relationship between single nucleotide polymorphisms in order to perinatal early intervention to reduce the incidence of hypoxic-ischemic encephalopathy and cerebral palsy caused by the hypoxic-ischemic brain disease. For the prevention and cure of neonatal hypoxic-ischemic encephalopathy find a better theoretical basisMethods 1. Study is divided into three groups: A group: neonatal hypoxic-ischemic encephalopathy 78 cases; B groups: normal control 100 cases; C group: neonatal hypoxic-ischemic encephalopathy caused 92 cases of children with cerebral palsy ; 2. Peripheral vein blood samples were drawn from the new-borns of each of the groups, from which genomic DNA was extracted respectively according to kit instructions. 3. Application of polymerase chain reaction (PCR) amplification of target gene 346bp, 4. PCR products of digestion and electrophoresis.Results 1. There was no significant difference in gestational age, birth weight, gender difference and feeding patterns among Group A, B and C (P>0.05). 2. A group of HIF-1αgene 1772CT genotype share more (30.77%),B, C group genotypes of HIF-1α gene 1772CT share less 18%, respectively, 18.57%.A, B, C three groups of HIF-1αgene C1772T genotype distribution was significantly different(P<0.05). HIF-1αgene C1772T / genotype group in the A group (mild, moderate and severe HIE) showed no significant difference(P>0.05). HIF-1αgene C1772T / genotype group in the C group (mild, moderate and severe HIE) showed a significant difference(P<0.05). 3. A, B group of HIF-1αgene G1790A genotypes do not meet Hardy-Weinberg equilibrium test. Therefore, A, B, C group HIF-1αgene G1790A polymorphism does not compare.Conclusion Hypoxic-ischemic encephalopathy/ cerebral palsy caused by the hypoxic-ischemic brain disease. and HIF-1αgene C1772T single nucleotide polymorphisms are associated.