The Relationship Between Kiss1/gpr54 And The Metastis Of Colorectal Carcinoma

Objective:1,Detection of Kiss1 in the colorectal carcinoma and the nodal metastases from advanced colorectal carcinoma, and investigate the relationship of Kiss1 with tumor metastis of colorectal carcinoma;2,Detection of GPR54 in the colorectal carcinoma and the nodal metastases from advanced colorectal carcinoma,and investigate the relationship of GPR54 with tumor metastis of colorectal carcinoma;3,Investigation the relationships of Kiss1 with GPR54 in tumor metastis of colorectal carcinoma.Methods:1,Expressions of Kiss1 were examined immunohistochemically with SP method in 121 specimens of colorectal primary carcinoma tissues and 61 specimens of nodal metastases from advanced colorectal carcinoma, and investigation the rel- ationship of Kiss1 with he colorectal carcinoma clinicopathological feature;2,Expressions of GPR54 were examined immunohistochemically with SP met- hod in 121 specimens of colorectal primary carcinoma tissues and 61 specimens of nodal metastases from advanced colorectal carcinoma, and investigation the relationship of GPR54 with he colorectal carcinoma clinicopathological feature;3,Investigation the relationships of the expression of Kiss1 with GPR54 in the colorectal carcinoma.Results:1,The expression of Kiss1 in colorectal carcinoma had no statistically significant relationships with patient's sex, age, pathology type, cancer embolus in vas, nerve infrige, cancer embolus in lymphatic, necrotic, nodal metastase and distant organize metastase.Kiss1 in the colorectal carcinoma which had not nodal metastase and which had nodal metastase positive rates were 71.67% and 52.46%, the difference had statistical significance(P<0.05); Kiss1 in the colorectal carcinoma which had not distant metastase and which had distant metastase positive rates were 69.00% and 28.57%,the difference had statistical significance(P<0.01);2,Kiss1 in the primary tumor tissues and the nodal metastases positive rates were 52.46% and 22.95%,the difference had statistical significance(P<0.01);3,GPR54 expression in colorectal carcinoma had no statistically significant roles with patient's sex, age, pathology type, cancer embolus in vas, nerve infrige, cancer embolus in lymphatic, necrotic, nodal metastase and distant organize metastase(P>0.05);4,GPR54 in the primary tumor tissues and the nodal metastases positive rates were 82.25% and 78.69%; the difference had no statistical significance(P>0.05);5,The expression of Kiss1 had no correlation with the expression of GPR54 (r=-0.146,P>0.05).Conclusion:1,Kiss1 positive rates in the colorectal carcinoma which had nodal metastase was lower than which had not nodal metastase , and also which had metastase was lower than which had not metastase; Kiss1 in the nodal metastases was also lower than the primary tumor tissues, so Kiss1 might play a critical role in the metastasis of color- ectal carcinoma, which suggested that Kiss1 might control the tumor metastis;2,GPR54 expression in colorectal carcinoma had no statistically significant roles with tumor clinicopathological feature, so GPR54 might not play the role in colorectal carc- inoma;3,The expression of Kiss1 had no correlation with the expression of GPR54,so Kiss1 played the roles in colorectal might not rely on GPR54. Objective:1,Construction the nude mice sub-skin model,orthotopic model(liver metastis model)transplanted with colorectal carcinoma which Kiss1 is positive and negative;2,Investigation the relationship of Kiss1 with metastis of colorectal carcinoma.Methods:1,Selection 17 colorectal carcinoma tissues which were positive and 9 colorectal carcinoma tissues which were negative, transplanted in the sub-skin; Than select one of the first sub-skin tumor which Kiss1 was postive, transplanted in the sub-skin and caecum again, also was which Kiss1 was negative. Finally, select one of the second sub-skin tumor which Kiss1 was posiive, transplanted in the caecum, constructed the orthotopic transplanted model(liver metastis model ), also was which Kiss1 was negative;2,The growth of tumor and the liver metastas were observed, then investigated the relaionships with Kiss1.Results:1,The nude mice sub-skin model,orthotopic model(liver metastis model) trans- planted with colorectal carcinoma which Kiss1 were positive and negative were constructed successfully;2,The successful rate of the first sub-skin model was 30.77%(8/26), the second was 68.75%(11/16), the difference had statistical significance(P<0.05); the secondary's growth delitescence was shorter than the first; the growth delitescence of Kiss1 negative is shorter ,but the bulk was bigger than that Kiss1 was positive, and the successful rate of the model which Kiss1 was negative was higher than the model which Kiss1 was positive(P<0.05);3,The successful rate of the second orthotopic transplanted model was 41.67%(7/16), the successful rate which Kiss1 was positive was 12.25%(1/8), the successful rate which Kiss1 was negative was 75.00%(6/8); The successful rate of the third orthotopic transplanted model was 75.00%(24/32), the successful rate of the model which Kiss1 was negative was higher than the model which Kiss1 was positive(P<0.05);4,The successful rate of the second liver metastis model was 28.57%(2/7), the third was 62.50%(15/24); the successful rate of the third orthotopic transplanted model which Kiss1 was negative was 80.00%(12/15), the successful rate which Kiss1 was positive was 33.33%(3/9);all the differences had statistical significance(P<0.05).Conclusion:1,The nude mice sub-skin model,orthotopic model (liver metatis model )transplanted with colorectal carcinoma were constructed successfully;2,The successful tate of the nude mice transplanted with colorectal carcinoma were higher than the next genetation;3,The successful rate of second sub-skin model and second,third orthotopic model transplanted with colorectal carcinoma which Kiss1 was negative were higher than that which Kiss1 was positive, suggest that Kiss1 might promote the growth of the transplanted tumor of colorectal carcinoma;4,The successful rate of the liver metastis model which Kiss1 was negative was higher than that which Kiss1 was positive, suggested that Kiss1 regulate the tumor metastis.