Isolation, Identification And Differentiation Of Rat's Muscle Satellite Cell

Precise voluntary movement in vertebrates is achieved by the fine coordinated control of skeletal muscles. The myofiber, the cellular unit of adult skeletal muscle, is a highly specialized syncytium sustained by hundreds of post-mitotic myonuclei. The repair and maintenance of this syncytium is conventionally assigned to a pool of undifferentiated myogenic precursors, satellite cells。Myoblasts have been widely used to regenerate skeletal and cardiac muscles and hold promise as a cell source for cell transplantation because they can easily be harvested from a patient's skeletal muscle and culture in vitro.Satellite cells are regarded as a population of muscle-specific committed progenitors that are responsible for the postnatal maintenance, growth, repair and regeneration of skeletal muscles. Although satellite cells were first isolated 50 years ago, the isolation protocols are still less efficient and needed to be modified. In this study, the combination of two-step enzyme digestion and purified with different speed adherence protocols were used to isolate rat satellite cells. Analyses of the cells with Desmin immunocytochemical staining and expressions of Myf5 and Myodl showed that they were skeletal muscle satellite cells. These cells can be induced into both myoblast and adipogenesis, which showed that these cells were pluripoent. These cells were used as recipients for transfection of fat-1 gene which derived from Worm (Caenorhabditis elegans).