Protective Effect Of Taur Our Sodeoxycholic Acid On The Apoptosis Of INS-1 Cells Cultured By Palmitate

Objectives:(1) To investigate the effect of palmitate on the apoptosis of INS-1cells in vitro.(2) To observe the effect of tauroursodeoxycholic acid (TUDCA) on the apoptosis of INS-1 cells induced by palmitate.(3) To investigate the probable mechanism by which tauroursodeoxycholic acid inhibits the apoptosis of INS-1 cells cultured with palmitate.Methods:(1) INS-1 cells were cultured in the absence or presence of different concentrations of palmitate (0.25mmol/L, 0.5mmol/L, 1.0mmol/L) for 12h. The survival rate of INS-1 cells was measured with methyl thiazolyl tetrazolium (MTT) method.(2) INS-1 cells were cultured in palmitate (0.5 mmol/L) and TUDCA (25μmol/L,50μmol/L,100μmol/L) for 12h. The survival rate of INS-1 cells was measured with methyl thiazolyl tetrazolium (MTT) method.(3) The apoptosis of INS-1cells was detected by flowcytometry (FCM).(4) Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) was performed to detect the gene expression of Bax/Bcl-2.Results:(1) The survival rate of INS-1 cells was decreased, while the apoptosis increased when the concentrations of palmitate were between 0.5mol/L and 1.0mmol/L (P<0.05).(2) The survival rate was increased, whereas the apoptosis decreased when TUDCA was added to INS-1 cells which were cultured with palmitate (0.5mmol/L) in the concentrations of 50μmol/L and 100μmol/L (P<0.05).(3) The expression of Bax gene was promoted, while Bcl-2 gene expression was depressed by palmitate (P<0.05). However, TUDCA could reverse such phenomenon.Conclusions:TUDCA can protect INS-1cells from apoptosis induced by palmitate because of reduced expression of Bax gene and enhanced expression of Bcl-2 gene.