The Study On Configuration Of Complex Of Quercetin-Mo And Its Biological Activities

Quercetin is a dietary flavonoid ubiquitous in nature, which has been shown to have many biological activities such as anti-inflammatory, antioxidant, antitumor activity, immunoregulation activity, scavenging free radicals and reducing the levels of sugar, lipid and press in blood. Molybdenum is the one of essential microelements, which has significant biophysical functions in vivo. There is a strong chelation between Quercetin and metal ions; therefore they are easy to compose. Inorganic biochemistry studies have shown that the biological activities of metal complexes are stronger than most of the precursors, but the side effects are smaller; even the complexes would have new functions. The reaction between quereetin and several metal ions is well known, such as quercetin and iron, zinc, chrome, cuprum, but little is known about the complex of quercetin and Mo. There has been study synthesized the complex of Quercetin-Mo, this research aim at making further study on its biological activities.Objectives1. To investigate the configuration of complex of Quercetin-Mo;2. To study the anti-mutagenic activities and the immunoregulation activities of the Quercetin-Mo complex;Methods1. To determine the structure and character of the complex of Quercetin-Mo through these methods of elemental analysis, thermal analysis, infrared spectrum and ultraviolet spectrum.2. To detect the growth inhibition rate of the complex of Quercetin-Mo on SMMC-7721 hepatoma cell, comparing the inhibition rate of various doses groups of Quercetin-Mo and Quercetin.3. To compare the effect of enhangcing the immunity of Quercetin-Mo and Quercetin by using experimental study of splenic lymphocyte proliferation of natural mice. 4. Through the experiments of the inhibiting effect of Quercetin-Mo to S-180 tumor-bearing mice with tumor models, and determine the SI of spleen and thymus, to discuss the anti-tumor effect and immunoregulation activity of Quercetin-Mo.5. Using the carbon clearance test and delayed-type hypersensitivity test (DTH) on mice, to study the role of Quercetin-Mo to enhance the immune and anti-tumor activity in normal mice.Results1. Combined with UV Spectral Analysis and the formation conditions of complex, conjecture that the reaction site of Quercetin and Molybdenum mostly involves 3-hydroxyl-4-ketone of cinnamyl. Compare with the infrared spectrum of Quercetin and Quercetin-Mo, find that there maybe water molecules participation in reaction. The further experiment by elemental analysis and thermal analysis show that 11 water molecules participation in reaction.2. Quercetin-Mo and Quercetin can inhibition the growth of SMMC-7721 hepatoma cell, and this effect has dose-effect relationship. Quercetin-Mo has a better anti-tumor activity than Quercetin (middle and low dose group P<0.05, high dose group P< 0.01); Quercetin-Mo can stimulate the proliferation of lymphocyte observably with dose-effect relationship.3. Quercetin-Mo can inhibition the growth of tumour in S-180 tumor-bearing mice, and with dose-effect relationship. Quercetin-Mo has significant stimulation to mice spleen with the dose of 0.69g·kg-1·d-1(P<0.01). Middle and high dose groups of Quercetin-Mo can enhance the action of T-lymphocyte observably(P<0.01). Various doses groups of Quercetin-Mo can promote the action of phagocytes, and middle dose group (0.46g·kg-1·d-1) with the best effet(P<0.01). Compare to blank control group various doses groups of Quercetin-Mo has significantly elevated the DTH reaction.Conclusions1. The study conjecture that the structural formula of Quercetin-Mo maybe C3oH22016Mo·11H20, molecular weight is 932 through a series of analyses.2. Quercetin-Mo has the activity of anti-tumor and immunoregulation in vivo and in vitro, and can significantly elevated the activity of mice immune competent cell.