The Activity Of NF-κB And Expression Of TNF-α MRNA In The PBMCs Of Renal Transplant Patients

The Nuclear factor-κB (NF-κB) was found in the nuclear extracts from B by Sen for the first time in 1986. It was binding protein that can bind to the gene enhancerκB specific sequence of the immunoglobulinκlight chain. NF-κB can combine inhibitory protein IκB into trimer in the cytoplasm in resting state. The activated NF-κB in the nucleus correspond target gene promoter or enhancer sequence and regulate the transcriptional activity of these genes based on the combination.Transplantation is the most effective treatment of end-stage renal, and it made a great progress in recent years. There was a higher survival rate after surgery because of the constant improvement in immunosuppressive therapy and the matures in surgical technology. However, The postoperative rejection and infection are not uncommon because of the administration based on weight. This may be associated with the individual's immunity level.Recent studies have found that, the activity of NF-κB is not only involved in tumor development, but also has become another target for cancer therapy. NF-κB as an important transcription factor, also plays an important function in the regulation of inflammation and immune responses. It has been confirmed that NF-κB is closely related with many inflammatory diseases, such as rheumatoid arthritis, asthma, atherosclerosis, Alzheimer disease. In organ transplantation, particularly renal transplant research, the activity of NF-κB in acute rejection after renal transplantation has been reported in animal studies. The research of NF-κB has not been reported in the infection patients after renal transplantation.TNF-a is the regulation factor of graft rejection, and can regulate a variety of inflammatory and immune response. In transplant rejection it has been recognized as an important factor, but the mechanisms have not been fully understood. The soluble TNF-a was detected by radioimmunoassay with plasma in the past, while the detection of intracellular TNF-a mRNA expression rarely has been reported.Objective:To study the activity of NF-κB and the expression of the tumor necrosis factor-a(TNF-a) in the peripheral blood mononuclear cells(PBMCs)in the renal transplant patients, the correlation NF-κB and TNF-a in renal transplant patients with the immune status of the individual level. To investigate whether the NF-κB and TNF-a are able to serve immune status indicators in the patients with renal transplantation.Methods:1 Obtain peripheral blood mononuclear cells (PBMCs) by density gradient centrifugation from the renal transplant patients and healthy control.2 The chemiluminescence electrophoretic mobility shift assay (EMSA) was applied to detected the activity of NF-κB in the peripheral blood mononuclear cells(PBMCs).3 Reverse transcription-polymerase chain reaction (RT-PCR) was applied to detected tumor necrosis factor-a(TNF-a) mRNA expression in the peripheral blood mononuclear cells (PBMCs).4 The SPSS17.0 statistical software was applied for the data statisties. The results were expressed with X±S. The analysis of variance (F test) or Kruskal-Wallis test was used to compare these data. test levelα= 0.05.Results:1 The postoperative stability group, infection group, rejection group patients after renal transplantation, The activity of NF-κB in the PBMCs are 4.65±1.37,16.43± 5.97,56.18±12.58, and the control group is 2.70±0.58. The postoperative stability group and the infected group, the stable group and the rejection group, the infection and the rejection group results are significantly different(P<0.05). The differences between the control group and the postoperative stability group are not statistically significant (P> 0.05).2 The postoperative stability group, infection group, rejection group patients after renal transplantation, the expression of TNF-a in the PBMCs were 0.445±0.12, 0.546±0.11,0.622±0.10, and the control group is 0.419±0.16. The stable group and the infected group, the postoperative stability group and the rejection group, the results are significantly different (P<0.05). The differences between control group and the postoperative stability group are not statistically significant(P>0.05). The differences between the infection and the rejection group are also not statistically significant (P> 0.05).Conclusions:1 The activity of NF-κB and the expression of TNF-a mRNA levels are significantly increased in the PBMCs when the rejection occurred after renal transplantation.2 The activity of NF-κB and the expression of TNF-a mRNA levels are significantly increased in the PBMCs when the infection occurred after renal transplantation. Compared with the rejection group, The activity of NF-κB have significant differences, while the TNF-a mRNA was not significant difference.3 The activity of NF-κB and the expression of TNF-a mRNA may exist nonlinear positive correlation, and they may be regard as immune status indicators for renal transplantation patients.