Treg Cells And Related Factors Affect On The Impact Of Embryo Repeated Implantation Failure

Objective:At present, many factors affect the embryo implantation, including intrauterine adhesions, hydrosalpinx, uterine shape abnormalities, hydrosalpinx, endometrial damaged.but treatment of these diseases would still rise to various high-quality transplants embryos have not yet pregnancy, known as the repeated implantation failure, or about 5%-10% ART. For all contribute of patients to the economy and spirit of the great blow, how to change the outcome of the treatment of these patients, improve planting success rate, become a reproductive experts and embryologist to explore a hot issue.CD4+CD25+regulatory T cells recently was recognized in a kind of important immune regulatory function of T cell subsets. Their dysfunction may be closely related to autoimmune diseases, and participate in the maintenance of peripheral T-cell homeostasis; they also play a key role in transplantation tolerance with the embryo implantation and early pregnancy maintenance. CD4+CD25+regulatory T cells development in regulation of the transcription factor FoxP3.using retro viral vectors to carry FoxP3 into naive CD4+T cells can achieve the latter to the CD4+CD25+ regulatory T cell transformation, indicating FoxP3 may be make CD4+CD25+regulatory T cell differentiation and function of the maintenance of important developmental regulatory gene. Adjustable-type T-cell factor:transforming growth factor-β, IL-10 regulates the differentiation of helper T cells involved in immune response and immune tolerance. The topics are designed to study the luteal phase as well as the ovum after six days which lays the number of CD4+CD25+regulatory T in peripheral blood, Foxp3, TGF-βin the expression of mRNA of the embryonic effects of repeated plant failures.Methods:1. The failure of the treatment of patients with repeated cultivation of 16 patients and the same period in the centers for the first cycle of IVF-ET treatment services in 25 cases served as controls were chosed from November 2009 to February 2010 at the First Affiliated Hospital of Zhengzhou University.2. urine LH test is measured Form the 12th day of menstrual cramps and appears positive after 6 days or menstrual cramps the first 22 days to 26 days (cycle 30-35 days), if the P value≥5ng/ml that the luteal phase were to collect blood. The same as 6 days after ovum collects of fasting blood 3ml, placed in EDTA anticoagulant tubes, which 2ml for Foxp3 and TGF-βlevels of mRNA detection, lml for flow cytometry detection3. Semi-quantitative RT-PCR assay detection of Foxp3 and TGF-βof the mRNA level in PBMCs; By flow cytometry in peripheral blood CD4+CD25high+CD127-Treg accounted for the ratio of CD4+T cells.Result:1. Two groups of age and fresh cycles were statistically significant difference (P <0.05), repeated cultivation of age and the failure group were significantly higher in fresh cycles. Men and women reasons unexplained infertility were no statistical differences between the two groups (P>0.05). The basis of menstrual cramps the first 2-4 days endocrine FSH, LH, E2 was no significant difference between the (P>0.05).2. Repeated planting failure group compared with control group, GnRH-a number of days, GnRH-a quantity, HMG number of days, HMG volume, E2 of HCG day, E2 of OPU day, OPU after 48h E2 have no statistically significant difference (P> 0.05). FSH dose of the two groups to start, FSH the number of days, FSH volume are statistically significant difference (P<0.05). The repeated failure of Group is significantly higher in FSH dosage and use of the number of days, as well as dose to star3. Repeated graft failure in peripheral blood d(LH+6), d(OPU+6) Foxp3mRNA are statistically significant difference compared with the control group (P<0.05).4. In Repeated planting failures and control groups of the d (LH+6), d (OPU+6) the number of Treg in peripheral blood volume is statistically different (P<0.05). The control group is higer than the repeated failure group.5. Repeated planting failure group than control group received fewer eggs, high-quality embryo rate, were statistically significant difference (P<0.05). Two sets of MⅡrate,2PN fertilization rate,2PN embryo cleavage rate, embryo utilization rate are not statistically different (P>0.05).6. The number of different periods of Treg are no statistically significant difference with matching data T test between the two group (8.14±1.87 vs8.24±1.49, P=0.547>0.05) in the d(LH+6), d(OPU+6).7. Treg number with age, number of cycles is negative correlation in d(LH+6) (P<0.05).respectively correlation coefficient is -.369,-.639; and Treg number with the number of retrieved oocytes, FSH start count, FSH days, FSH support a few, HCG day E2, OPU day E2, OPU48 hours of E2 were not correlated (P>0.05).8. Treg number with age, number of cycles is negative correlation in d(LH+6) (P <0.05). The correlation coefficient is -.324 and -.639.9.In the case of controlling for age, d (LH+6), d (OPU+6) Treg number and number of cycles make partial correlation analysis (P<0.05).Respectively, the partial correlation coefficient is -.686,-.578.10. The peripheral blood d(LH+6), d(OPU+6) TGF-βmRNA of the repeated cultivation of failure are significantly different than the control group (P<0.05).Conclusion:1. Repeated cultivation of peripheral blood of patients failed to reduce significantly the number of Treg cells and Foxp3 expression of genes related to reduction may be repeated cultivation of the reasons for failure of immune infertility.2. Controlled ovarian hyperstimulation of the medication will not affect the number of Treg cells.3. In the controlled ovarian hyperstimulation of the E2 levels do not affect the number of Treg cells in peripheral.4. The peripheral Treg cells are related factors with age and the cycle increases, reducing the number of peripheral Treg cells.5. Immunosuppressive factor TGF-βin the peripheral blood of patients fail cultivated in reduced expression, which is their immunological infertility causes.